青藏高原近缘野生大麦5SrRNA基因染色体原位杂交定位  

CHROMOSOMAL LOCALIZATION OF 5S rRNA GENES IN WILD RELATIVES OF BARLEY FROM QING-ZANG PLATEAU BY IN SITU HYBRIDIZATION

作　　者：闫玲[1] 丁毅[1] 陈新平 宋运淳[1] 方呈祥 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2000年第6期443-448,T001,共7页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金! ( No.39670 0 51 );国家教委博士点基金

摘　　要：采用原位杂交技术 ,以 5S r RNA基因为探针 ,对产于青藏高原的 4份近缘野生大麦和栽培大麦 ,即 :二棱野生大麦 H ordeum vulgare L.ssp.spontaneum(Koch) Hsü,六棱野生大麦 H.vulgare L.ssp.agriocrithon(A○ berg) Hsü,六棱瓶形野生大麦 H.vulgare L.ssp.agriocrithon var.lagunculiform(Bakht) Hsü,栽培大麦 H.vulgare.L.进行了研究 ,将杂交结果进行观察与统计 ,并建立起 5S r RNA基因定位的模式图。结果表明 5S r RNA基因在染色体上的位点呈现动态变化 ,由二棱野生大麦、六棱瓶形野生大麦到六棱野生大麦、栽培大麦 ,位点数目有递增的趋势 ,而且位置也发生了某些改变。探讨了 5Sr RNA基因进化与大麦染色体进化间的关系以及原位杂交技术在系统进化研究中的应用。Four representatives of wild relative and cultivar of barley,i.e.two rowed wild barley Hordeum vulgare L.ssp. spontaneum (Koch) Hsü, six rowed wild barley H.vulgare L.ssp.agriocrithon (A○berg) Hsü,six rowed bottle shaped wild barley H.vulgare L.ssp. agriocrithon var.lagunculiform (Bakht) Hsü and cultivated barley H.vulgare L.ssp.vulgare Hsü from Qing Zang Plateau were studied by in situ hybridization using 5S rRNA genes as probe.Located 5S rRNA genes sites were used to construct an idiogram,which provided as molecular karyotype.It showed that 5S rRNA genes in barley were movable taking into account of its localization and, total number of sites for 5S rRNA genes increased from two rowed wild barley,six rowed bottle shaped wild barley to six rowed wild barley and cultiva ted barley.The relationship between evolution of 5S rRNA genes and that of barley as well as the application of in situ hybridization in phylogeny were discussed.

关 键 词：近缘野生大麦 5SrRNA基因 原位杂交 系统发育 

分 类 号：S512.301[农业科学—作物学]