植物油降低心脏ω-6/ω-3多不饱和脂肪酸比值并改善心肌缺血再灌注的研究  

Plant oil intake lower ω-6/ω-3 polyunsaturated fatty acids ratio in the heart and improve the ischemia/reperfusion injury and the expression of CYP2J

作　　者：张晓霞[1] 米树华[1] 李斌[1] 潘晓东[2] 赵子建[3] 戴一凡[3] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所特需医疗科,北京100029 [2]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所,北京100029 [3]南京医科大学

出　　处：《心肺血管病杂志》2013年第6期773-776,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：北京市自然科学基金(7112040)

摘　　要：目的:探讨适当增加饮食中植物油摄入,ω6/ω3多不饱和脂肪酸(polyunsaturated fatty acids,PUFAs)比例,及其对心肌缺血-再灌注(ischemia-reperfusion,I-R)时,细胞色素P450表氧化酶(cytochrome P450-enzyem)CYP2J表达及心肌损伤程度的影响。方法:C57BL/6小鼠80只,随机分为普通饮食组及植物油组。分别以相应饮食喂养1个月后,建立心脏缺血再灌注模型,TTC染色检测心肌梗死面积。实时聚合酶链反应(PCR)及Western blot检测心肌CYP2J表达。气象色谱法检测细胞膜ω-3 PUFAs、ω-6 PUFAs及环氧二十碳三烯酸(11,12-epoxyeicosatrienoic acid,EET)含量。结果:植物油组小鼠心脏ω-6/ω-3比值显著低于普通饮食组,其缺血区心肌CYP2J表达及EET含量显著高于普通饮食组,心肌梗死面积显著改善。结论:适当增加饮食中植物油摄入降低心脏ω-6/ω-3比值,改善心肌缺血再灌注时CYP2J表达,改善心肌缺血再灌注损伤。Objective：To investigate how does the addition of plant oil in the diet influence the ω-6/ω- 3 PUFAs ratio in the heart and the effect of those on the expression of cytochrome P450 2J（CYP2J） and the my- ocardial injury due to ischemia/reperfusion. Methods： 80 C57BL/6 mice, randomly divided into ordinary diet group and plant oil group. One month later, myocardial ischemia/reperfusion model was built in these mice, 2, 3,5 triphenyltetrazolium chloride （TTC） staining was used to measure infarction size. Real time reverse tran- scription-polymerase chain reaction（RT-PCR） and Western blot was used respectively to detect the mRNA and protein expression of CYP2J. ω-6/ω-3 PUFAs and EET were tested by high-performance gas chromatography. Results： ω-6/ω-3 PUFAs ratio in the heart decreased in the plant oil group than in the ordinary diet group, af- ter ischemia/reperfusion, the expression of CYP2J and the amount of EET in the area at risk was higher in the plant oil group, Infarction size of plant oil group were diminished. Conclusion ： Properly increase plant oil in- take lower the ω-6/ω-3 PUFAs ratio, improve the expression of CYP2J and the ischemia/reperfusion injury.

关 键 词：Ω-3多不饱和脂肪酸 缺血-再灌注损伤 细胞色素P450表氧化酶 动物试验 小鼠 

分 类 号：R54[医药卫生—心血管疾病]