水稻叶色白化转绿及多分蘖矮秆突变体hfa-1的基因表达谱分析  

Gene Differential Expression of a Green-Revertible Albino and High-Tillering Dwarf Mutant hfa-1 by Using Rice Microarray

作　　者：郭涛[1] 黄永相[1] 罗文龙[1] 黄宣[1] 王慧[1] 陈志强[1] 刘永柱[1] 

机构地区：[1]华南农业大学/国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《作物学报》2013年第12期2123-2134,共12页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31200250);国家高技术研究发展计划(863计划)项目(2012AA101201);广东省自然科学基金项目(S2011010001892)资助

摘　　要：hfa-1的白化转绿、多分蘖矮秆表型受单隐性核基因hw-1(t)控制。该基因编码含线粒体交替氧化酶AOX结构的叶绿体蛋白,通过参与叶绿体呼吸电子传递链,对类胡萝卜素生物合成途经及其次生代谢途径进行调控。本研究对hfa-1突变体叶色白化转绿过程中的类胡萝卜素生物合成途径、与该途径有关的植物激素代谢途径的相关基因进行表达分析,并分析了hfa-1突变体在白化、转绿2个时期的基因表达谱。结果表明,类胡萝卜素、植物激素(GA、ABA和SL)生物合成相关基因在hfa-1突变体白化期叶片中表达量减低,暗示hw-1(t)基因的突变抑制了苗期类胡萝卜素合成,同时还对涉及该过程相关多个次生代谢途径产生影响。通过基因表达谱芯片和功能分类分析,发现hfa-1叶色白化转绿过程中上调和下调表达基因涉及的生理过程主要在光合作用、应对内源刺激物和胁迫响应等方面;其中电子传递体Cytb6/f复合蛋白合成相关基因上调表达明显,推测Cytb6/f蛋白复合体在电子传递和质醌库还原氧化上对hw-1(t)起一定的补偿功能。Phenotype of the hfa-1 mutant characterized by green-revertible albino and high-tillering dwarf is controlled by a single recessive nuclear gene hw-1（t）, which encodes a chloroplast protein containing the mitochondrial alternative oxidase （AOX） structure and involves in the electron transport chain of chloroplast respiratory regulating biosynthesis of carotenoid and carotenoid-related secondary metabolic pathways. In this study, expression analysis of genes involved in carotenoid biosynthesis and carotenoid-related plant hormones biosynthesis andgene-expression profiling were conducted before and after the turning-green of hfa-1 mutant. the results displayed that expression levels of genes involved in carotenoid biosynthesis and carotenoid-related plant hormones （GA, ABA and SL） biosynthesis were decreased in the albino leaves of hfa-1, suggesting that mutation of hw-1（t） inhibits the biosynthesis of carotenoid and has an impact on carotenoid-related secondary metabolic pathways. Gene expression profiling and functional classification showed that genes related to physiological processes in response to photoeynthesis, endogenous stimulus and stress were differently expressed in hfa-1 before and after its turning-green. Expression of genes encoding proteins related to the electron transport complex Cytb6/f were up-regulated significantly, indicating that Cytb6/f would provide some compensation in electron transport and redox of plastoquinone in the hfa-1 mutant.

关 键 词：水稻 白化转绿 突变体hfa-1 基因表达分析 

分 类 号：S511[农业科学—作物学]