沙棘多糖的提取纯化及其对巨噬细胞的作用研究  

EXTRACTION AND PURIFICATION OF SEABUCKTHORN POLYSACARIDE AND EFFECTS ON MACROPHAGES

作　　者：宋亮[1] 张威[1] 董仕超 谢基明[2] 王玉珍[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古自治区医院检验科,呼和浩特010010

出　　处：《内蒙古农业大学学报（自然科学版）》2013年第5期87-92,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(81260662;31270922);内蒙古自然科学基金(2010MS0513;2011MS1112;20121156);内蒙古科技厅应用技术研发计划项目(20070501)

摘　　要：本文以内蒙古大果沙棘为材料,用超声裂解法结合热水提取法提取沙棘多糖。并用Sevage法结合木瓜蛋白酶法除蛋白,SephadexG-150进行柱层析,提取并纯化出沙棘多糖冻干纯品HPRⅠa,并对其进行纯度鉴定。用MTT法和中性红法初步探讨沙棘多糖对RAW264.7巨噬细胞的作用。发现随着多糖作用浓度的增加,RAW264.7细胞的增殖活性和吞噬能力也随之增强,作用浓度达到300μg/mL时细胞的增殖活性基本达到一个平台期。由此可已初步判断HPRⅠa对巨噬细胞具有明显的促增殖作用。Hippophae rhamnoides of Inner Mongolia is used as the material. Seabuekthorn polysaeeharide was extracted by combining ultrasonic treatment and hot water extraction methods. Protein was removed by combining sevage method and papain hydrolytic method. The lyophilized seabuckthorn polysaccharide （HPR [ a） was first purified by Sephadex G - 150 column chromatography, and then i- dentified for its purity. MTr assay and neutral red methods were used to study the effect of seabuckthorn polysaccharide on RAW264. 7 macrophages. We found that seabuckthorn polysaccharide enhanced the proliferative activity and phagocytosis of RAW264. 7 in a con- centration - dependent manner. The proliferative activity arrived at a plateau phase when the concentration of seabuckthorn polysaccha- ride reaches at 300μg/mL. We conclude that HPR l a promotes the proliferation and phagocytosis of RAW264. 7 macrophages.

关 键 词：沙棘多糖 提取 纯化 巨噬细胞 增殖 

分 类 号：R967[医药卫生—药理学]