猪囊虫病基因工程疫苗用于免疫治疗的研究  

Immunotherapy for Swine Cysticercosis with the Genetic Engineering Vaccine

作　　者：唐雨德[1] 顾志香[1] 刘玉[1] 周宗安[1] 栾维民[2] 刘德惠 王明珠 

机构地区：[1]南京军区军事医学研究所,南京210002 [2]吉林农业大学动物医学系 [3]内蒙古自治区科右前旗兽医工作站

出　　处：《中国预防兽医学报》2000年第6期436-439,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：应用猪囊虫病基因工程疫苗分别对实验性猪囊虫病和自然感染的猪囊虫病进行分组治疗 ,以探讨该疫苗的免疫治疗作用。 10头人工复制的囊虫病猪 ,随机分成 3组。第 1组 4头猪 ,于感染后 1个月注射该疫苗 ,连续 3次 ,间隔 15天 ;第 2组 4头猪 ,于感染后 2个月注射疫苗 ,同上连续 3次 ;第 3组 2头猪 ,不注苗。第 1组注射疫苗后 1个月循环抗原滴度开始降低 ,2 .0、2 .5及 3 .5个月各有 1头猪CA转阴 ,5个月OD值分别为 0 .14、0 .0 9、0 .17、0 .38。第 2组和第 3组各猪血清OD值一直维持在 1.0以上。感染后 5个月剖检 ,第 1组 4头猪均末检出囊虫 ;第 2组 4头猪在咬肌、膈肌、腰肌、股内侧肌、肩胛外侧肌等部位均检测到囊虫 ,检测部位 40cm2 面积发现囊虫 3～ 7个 ,有部分虫体已钙化 ,胆汁孵化率为 15 .4% ;第 3组各猪也在各检测部位检测到囊虫 ,40cm2 的囊虫数为 6～ 9个 ,胆汁孵化率 90 .4%。 2 5头自然感染的囊虫病猪 ,按OD值大小分 3组进行免疫治疗 ,连续注射疫苗 3次 ,每次间隔 15天 ,6个月后剖检。OD值在 0 .6 8～ 0 .90的 7头猪均未检测到囊虫 ,OD值在 0 .91～ 1.10的8头猪和OD值在 1.11～ 1.5 4的 10头猪分别有 5头和 2头猪未检测到囊虫。In order to probe the effects of the immunotherapy for swine cysticercosis with the genetic engineering vaccine,the pigs infected experimentally or naturally with cysticercosis were treated with the vaccine. 10 pigs which were infected experimentally cysticercosis were randomly divided into three groups: l)four pigs,each was injected the genetic engineering vaccine against swine cysticercosis after one month of infection for three times at interval of fifteen days; 2)four pigs,each was injected the vaccine as above after two months of infection; 3)two pigs,each was injected nothing.The sera CA of all the infected pigs were positive,which were detected by sandwich ELISA,and their OD values were between 0.9 and 1.42 after one month of infection.In the first group,the OD values began to decrease in one month of the first injection of the vaccine,and one of the CAs was negative in 2.0,2.5and 3.5 months,respectively,no cysticerci was found.In the second group,cysticerci was found in the detected muscles of all the four pigs:masseter,septonles,psoas,vastus medialis and scapulae externus.The number of cysticerci hosted in the detected pork was 3 to 7 per 40 cm 2.Their bile hatching rates were 15.4%.In the third group,the cysticerci were detected in the two pigs,the number of cysticerci hosted in the detected pork was 6 to 9 per 40 cm 2,their bile hatching rates were 90.4%.By epidemic investigation detecting the sera's CA,we confirmed that 25 pigs were infected naturally with cysticercosis.They were divided into three groups as their OD values and were injected with the genetic engineering vaccine as above: 1)7 pigs,their OD values between 0.68 and 0.90; 2)8 pigs,their OD values between 0.91 and 1.10; 3)10 pigs,their OD values between 1.11 and 1.54.Six months later,no cysticerci was found in all the pigs of the first group,in 5 pigs of the second group and in 2 pigs of the third group,respectively.These results revealed that the genetic engineering vaccine could play important role in the therapy to swine cysticercosi

关 键 词：猪 囊虫病 基因工程疫苗 免疫治疗 疗效试验 

分 类 号：S858.285.9[农业科学—临床兽医学] S858.282.5[农业科学—兽医学]