参附滴丸中皂苷类成分的HPLC指纹图谱研究  

HPLC Fingerprint of Saponins in SHENFU Dropping Pills

作　　者：柳君君[1] 张春椿[1] 李效贤[1] 姚静[1] 熊耀康[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中医药大学学报》2013年第11期1279-1283,共5页Journal of Zhejiang Chinese Medical University

基　　金：浙江省自然科学基金重大项目(Z2091224)~~

摘　　要：[目的]建立参附滴丸、中间体浸膏中皂苷类成分的HPLC指纹图谱,为科学评价和有效控制参附滴丸的质量提供分析方法。[方法]采用Hypersil BDS C18柱(250 mm×4.6 mm,5μm)色谱柱,柱温25℃,流动相为乙睛、水,梯度洗脱,流速1.0mL·min-1,检测波长203nm,对参附滴丸、中间体浸膏中皂苷类成分分别进行指纹图谱测定,并比较分析两者之间的相似度及相关性。[结果]初步建立了10批参附滴丸和中间体浸膏中皂苷类成分的HPLC指纹图谱,其中滴丸确定了11个共有峰,而中间体浸膏则16个共有峰,中间体浸膏比滴丸多出5个共有峰,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,指纹图谱相似度均在0.9以上。[结论]所建立的HPLC指纹图谱方法具有较好的重复性,为参附滴丸的质量评价提供了有效的分析方法。[Objective]The fingerprint chromatograms were established for quality evaluation of saponins in SHENFU Dropping Pins and intermediate products by HPLC,thns to provide the analysis method for quality evaluation and quality control of the SHENFU Dropping PiUs.[Method]The analysis was performed on a Hypersil BDS C18 column （250mm×4.6mm,5μm） at 25℃ with the mobile phase consisted of acetonitrile and water for gradient elution with the flow rate at 1.0mL·min^-1. The detection of wavelength was at 203nm. Similarity and correlation of the fingerprints of the saponins in SHENFU Dropping Pills and intermediate products were analyzed. [Result]Under the above chromatographic conditions, HPLC fingerprint of saponins in 10 batches SHENFU Dropping Pills and 10 batch intermediate products was set up. 11 common peaks were found from SHENFU Dropping Pills and 16 common peaks were found from intermediate products. Intermediate products had more 5 common peaks than SHENFU Dropping Pills. The relative standard deviations for relative retention time and relative peak areas were less than 3% in the precision and repeated test. All of the similarity of samples was above 0.9.[Conclusion]The establishment of HPLC fingerprint had high repeatability. The method could be used for the quality evaluation of SHENFU Drop- ping Pills.

关 键 词：参附滴丸 中间体浸膏 皂苷 HPLC 指纹图谱 

分 类 号：R245.9[医药卫生—针灸推拿学]