利用STS标记对家蚕雏翅基因mw的定位分析  被引量:3

Mapping Analysis of Minute Wing Gene( mw ) in Silkworm,Bombyx mori by Using STS Markers

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作  者:马晓[1] 刘先方[2] 易晓莉[1] 侯成香[2,3] 李冰[2] 李木旺[2,3] 

机构地区:[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]江苏科技大学蚕业研究所,江苏镇江212018 [3]中国农业科学院蚕业研究所,江苏镇江212018

出  处:《蚕业科学》2013年第6期1104-1107,共4页ACTA SERICOLOGICA SINICA

基  金:国家高技术研究发展计划"863"项目(No.2013AA10-2507);江苏省六大人才高峰项目(No.NY-025)

摘  要:家蚕成虫翅膀的飞行能力已经退化,利用分子标记技术对翅突变基因进行定位分析,可为家蚕翅发育调控机制研究以及鳞翅目害虫的生物防治提供有参考价值的信息。采用家蚕正常翅形品种P50和雏翅(minute wing,mw)品种u11为亲本组配正反交群体,并获得回交群体(P50×u11)♀×u11♂和u11♀×(P50×u11)♂,分别记作BC1F和BC1M,用STS分子标记方法对mw基因进行定位。由于雌性家蚕的染色体不会发生交换,利用BC1F群体筛选与mw基因连锁的分子标记,并用BC1M群体构建mw基因的STS分子标记遗传连锁图,筛选到与mw距离最近的STS标记T2272,其与mw的遗传距离为1.1 cM。The flying ability of silkworm ( Bombyx mori) has been degenerated. Mapping analysis of wing mutational genes by molecular marker technology may provide useful information for exploration of regulatory mechanism of silkworm wing development and biocontrol of lepidopteran pests. In this research, silkworm variety P50 which has normal wing and silkworm variety ull which has minute wing (mw) were used as parents to prepare reciprocal backcross populations. Two back-crossed populations ( P50 × u11 ) ♀ × ull ♂ and ull ♀ ×( P50 × ull )♂ , designated as BC1 F and BC1M respectively, were obtained. Subsequently, mw gene was mapped using STS molecular markers. Owing to the lack of crossing over in female silkworm, the molecular markers linked with mw gene were confirmed by utilizing BC1F population. The other population, BC1 M, was used to construct the genetic linkage map between mw and STS markers. The STS marker T2272 which has the closest qenetic distance, 1.1 cM, with mw was obtained.

关 键 词:家蚕 雏翅 基因定位 STS标记 

分 类 号:S881.2[农业科学—特种经济动物饲养]

 

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