植物生长调节剂芸苔素内酯对家蚕的毒性试验  

Toxicity Test of Plant Growth Regulator Brassinolide to Bombyx mori

作　　者：李前龙[1] 沈中元[1,2] 唐旭东[2] 徐莉[2] 燕薇[1] 朱峰[1] 张姣[1] 侯建革[1] 

机构地区：[1]江苏科技大学,江苏镇江212003 [2]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《蚕业科学》2013年第6期1121-1125,共5页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系专项(No.CARS-22);"十二五"国家科技支撑计划项目(No.2011BAD33B04)

摘　　要：芸苔素内酯是一种新型的植物生长调节物质。采用食下毒叶法测定芸苔素内酯对家蚕的毒性,并研究其对家蚕生长发育的影响,为套种模式桑园使用芸苔素内酯对家蚕的安全性评价提供依据。芸苔素内酯对家蚕2龄幼虫经口急性毒性的半数致死浓度(LC50)值为3.411 8μg/mL;0.001、0.010和0.100μg/mL芸苔素内酯添食组的家蚕幼虫全龄经过时间较对照组分别延长2 d 18 h、4 d 6 h和5 d 12 h,全茧量、茧层率和茧层量较对照组显著或极显著降低,且对雄蚕茧层率的影响更为明显;用0.100、1.000μg/mL芸苔素内酯添食后家蚕幼虫全龄期的累计死亡率分别达80.50%和100%。研究结果表明,芸苔素内酯对家蚕的生长发育和产茧性状均有不良影响,并具有添食浓度效应:低剂量(0.001和0.010μg/mL)芸苔素内酯表现为延缓家蚕幼虫的生长发育;高剂量(0.100和1.000μg/mL)芸苔素内酯对家蚕有明显的致死作用。Brassinolide is a novel plant growth regulator. In order to provide references for safety evaluation of using brassinolide in mulberry field with intercrops, the toxicity of brassinolide to silkworm （ Bombyx mori） was determined in laboratory through feeding the larvae with poisoned mulberry leaves, and the influence of brassinolide to silkworm growth and development was investigated. It was found that the LC50 of brassinolide acute toxicity via oral intake by the 2nd instar larvae was 3. 411 8 μg/mL. After intaking 0. 001, 0. 010 and 0. 100 μg/mL brassinolide, the duration of all instars was prolonged by 2 d 18 h, 4 d 6 h and 5 d 12 h, respectively. Cocoon weight, cocoon shell rate and cocoon shell weight were all lower than the control group. Besides, the influence to cocoon shell rate of male larvae was more obvious than that to female ones. The all-instar mortality rate of silkworm larvae reached 80.50% and 100% respectively after 0. 100 and 1. 000μg/mL brassinolide were taken in by the larvae. The results indicated that brassinolide has obvious harmful effects to silkworm growth, development and cocoon characters, and the effect is dependent on dose of brassinolide applied. Brassinolide of low dose （0. 001 and 0. 010 μg/mL） delays the growth and development of silkworm larvae, while that of high dose （0. 100 and 1. 000 μg/mL） has remarkable lethal effect to silkworm larvae.

关 键 词：芸苔素内酯 家蚕 半数致死浓度 生长发育 产茧性状 

分 类 号：S884.96[农业科学—特种经济动物饲养]