宫内生长受限大鼠肝组织胰岛素样生长因子-2与过氧化物酶体增殖物激活受体-γ辅激活因子-1α表达的相关性  

Relation between expression of insulin-like growth factor-2 and peroxisome proliferator-activated receptor-γ coactivator-1α in fetal growth restricted rats

作　　者：张美慧[1] 廖立红[1] 谢雪梅[1] 周秀云[1] 叶娟[1] 应艳琴[1] 宁琴[1] 罗小平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《中华围产医学杂志》2013年第12期759-765,共7页Chinese Journal of Perinatal Medicine

基　　金：国家自然科学基金（81170627）;长江学者和创新团队发展计划（PCSIRT1131）

摘　　要：目的 探讨胎儿生长受限（fetal growth restriction,FGR）仔鼠生后追赶生长导致成年期代谢综合征的发生机制.方法 孕Sprague Dawley大鼠随机分为正常饮食组和限制饮食组,各10只.限制饮食组孕鼠摄食量为正常饮食组孕鼠摄食量的30％,建立FGR仔鼠模型.限制饮食组和正常饮食组孕鼠妊娠19d时,取胎盘和胎鼠肝组织.限制饮食组新生仔鼠体重低于正常饮食组仔鼠体重平均值2个标准差者定义为FGR仔鼠.取新生仔鼠、4及8周龄完成追赶生长的仔鼠的肝组织,采用实时荧光定量-聚合酶链反应及Western印迹技术检测胎盘及不同周龄仔鼠肝组织胰岛素样生长因子-2（insulin-like growth factor-2,IGF-2）、磷脂酰肌醇3-激酶（phosphatidylinositol 3 kinase,PI3-K）、蛋白激酶（serine/threonine protein kinases,AKT2）、磷酸化AKT2 （phosphorylation of AKT2,AKT2-P）和过氧化物酶体增殖物激活受体-γ辅激活因子-1α（peroxisome proliferator-activated receptor-γ coactivator 1α,PGC-1α）mRNA及蛋白表达水平.测定8周龄完成追赶生长的FGR仔鼠和对照组仔鼠血浆甘油三酯浓度,并取新鲜肝组织,HE染色和油红O染色观察肝组织病理改变.采用t检验和Pearson直线相关分析进行统计学分析.结果 FGR仔鼠胎盘、胎肝、新生肝、4周肝组织中IGF 2 mRNA的表达水平分别为0.665±0.210、7.855±0.354、6.714±0.744和（4.510±0.490）×10-4,均低于对照组[分别为1.608±0.464、9.548±0.416、13.053±1.415和（22.800±0.060）×101]（t分别为4.535、7.175、9.713和4.580,P均＜0.01）.FGR仔鼠胎盘、胎肝、新生肝组织中PI3-K、AKT2、AKT2 P和PGC-1α的蛋白表达水平均分别较对照组显著降低（P均＜0.01）.8周龄仔鼠肝组织内IGF-2 mRNA表达检测不出,但PI3-K、AKT2、PGC hα mRNA的表达水平分别为0.380±0.150、1.020±0.019和0.280±0.160,均显著低于对照组（分别为1.510±0.220、2.360±0.360和0.680±0.350）（t分别为14.700、12.876和3.Objective To explore the potential mechanism of metabolism syndrome formation in fetal growth restriction （FGR） rats with catch-up growth （CG-FGR）.Methods Pregnant SpragueDawley rats were randomly divided into normal nutrition group and nutrition restriction group,10 rats in each.The food intake of nutrition restriction group was 30％ of that of normal nutrition group,so as to establish the FGR rat model.Three pregnant rats in each group were sacrificed at their 19th gestational day,the placentas and fetal livers were collected.The newborn rats in nutrition restriction group whose birth weights were two standard deviations lower than the mean birth weights of normal nutrition group were defined as FGR rats.The liver of newborn rats,4-week and 8-week CG-FGR rats were collected.Real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein levels of insulin-like growth factor-2 （IGF-2）,phosphatidylinositol 3-kinase （PI3-K）,serine/threonine protein kinases-2 （AKT2）,phosphorylation of AKT2 （AKT2-P） and peroxisome proliferator-activated receptor-γ coactivator-1α （PGC-1α） in placenta and liver tissues.The plasma triglyceride levels of CG-FGR rats and normal rats at 8-week were detected,and their livers were stained by HE and Oil red O to observe the pathological changes.Pearson linear correlation analysis and t-test were applied for statistics.Results IGF-2 mRNA levels of FGR rats （as in placenta,fetal liver,newborn liver and 4-week rat liver） were lower than those of the control rats [0.665±0.210 vs 1.608± 0.464,t =4.535; 7.855 ± 0.354 vs 9.548 ± 0.416,t =7.175; 6.714 ± 0.744 vs 13.053±1.415,t=9.713; （4.510±0.490）×10-4 vs （22.800±0.060）×10 4,t=4.580;all P＜0.01].The protein expressions of PI3 K,AKT2,AKT2-P and PGC-1α of FGR rats in placentas,fetal livers and newborn livers were lower than those of control rats （P＜0.01,respectively）.Although IGF2 mRNA levels in 8-week rats＇ liver were detected in neither groups,the mRNA level

关 键 词：胎儿生长迟缓 胰岛素样生长因子2 转录因子 RNA结合蛋白质类 代谢综合征X 

分 类 号：R714.5[医药卫生—妇产科学]