静脉免疫球蛋白对早产儿脐血T淋巴细胞功能的影响  

An in vitro study on influence of intravenous immunoglobulin on function of T cells in cord blood of preterm infants

作　　者：常红[1] 张秋业[1] 庄超 盛红玲[3] 吴蒙蒙[1] 朱海燕[1] 

机构地区：[1]青岛大学医学院附属医院儿内科,266003 [2]青岛市儿童医院血液科 [3]山东省交通医院儿科

出　　处：《中华围产医学杂志》2013年第12期766-769,共4页Chinese Journal of Perinatal Medicine

摘　　要：目的 观察不同剂量静脉免疫球蛋白（intravenous immunoglobulin,IVIG）对早产儿脐血T淋巴细胞功能的影响,探讨IVIG免疫调节作用的机制.方法 选择2011年6月1日至2011年12月31日在青岛大学医学院附属医院出生、胎龄32～34周的早产儿15例为研究对象.在无菌条件下采用密度梯度离心法获取脐血单个核细胞（cord blood mononuclear cells,CBMC）,置于不同浓度IVIG（A、B、C组分别为6、9和12 mg/ml）条件下体外培养72h.采用流式细胞仪检测CD4＋CD25＋ Foxp3＋调节性T淋巴细胞（regulatery T lymphocyte,Treg细胞）的表达率,酶联免疫吸附法检测培养上清液中干扰素（interferon,IFN）γ、白细胞介素（interleukin,IL）4、IL-10和转化生长因子（transforming growth factor,TGF）-β1的水平.采用单因素方差分析和LSD检验进行统计学处理.结果 （1） CBMC分泌IFN-γ及IL-4水平变化：C组CBMC分泌IFN 7水平为0.03±0.02,低于A组的0.18±0.08和B组的0.13±0.05（F=5.72,P＜0.01）,而A组与B组差异无统计学意义（P＞0.05）;B组和C组CBMC分泌IL-4水平（分别为0.02±0.01和0.01±0.01）均显著低于A组的0.03±0.01（F=20.38,P均＜0.01）,B组与C组差异无统计学意义（P＞0.05）;（2）各组Treg细胞的表达率差异均无统计学意义（F=0.67,P均＞0.05）.（3）C组CBMC分泌IL-10水平为3.02±3.79,明显低于B组（6.90±4.64）和A组（10.78±5.44）（F=4.68,P＜0.05和0.01）;B组也明显低于A组（P＜0.05）;B组（16.15±13.94）和C组（8.44±13.71）CBMC分泌TGF-β1水平均显著低于A组（30.23±16.32）（F=5.22,P＜0.05和0.01）,B组与C组差异无统计学意义（P＞0.05）.结论 IVIG可抑制早产儿脐血辅助性T淋巴细胞1和2的细胞功能,且与其剂量有关;IVIG可通过调节细胞因子IL 10和TGF-β1的分泌抑制天然脐血Treg细胞的功能,且有剂量依赖效应.Objective To observe the influence of different doses of intravenous immunoglobulin （IVIG） on function of T cells in cord blood of premature infants and to explore the immunomodulating mechanism of IVIG.Methods Cord blood mononuclear cells （CBMC） were isolated by density gradient centrifugation method from 15 preterm infants born between June 1,2011 to December 31,2011 at 32 34 gestational weeks.Three groups were formed according to the concentrations of IVIG used for CMBC culturing in vitro （Group A,6 mg/ml; Group B,9 mg/ml; Group C,12 mg/ml）.After 72 hours in sterile conditions,the levels of IFN 7,IL 4,IL-10 and TGF β1 in the supernatant were determined by enzyme-linked immunosorbent assay.The expression of CD4＋ CD25＋ Foxp3＋ Treg cells was examined by fluorescent activated cell sorter.Differences among groups were compared by one way analysis of variance.For comparison between two groups,LSD test was used.Results （1） The level of IFN γ secreted by CBMC in Group C was 0.03 ± 0.02,which was much lower than that in Group A（0.18±0.08） and Group B（0.13±0.05） （F=5.72,both P＜0.01）.The level of IFN-γ in Group B and Group A did not show statistical difference （P＞0.05）.Compared with Group A（0.03±0.01）,the level of IL4 was much lower in Group B （0.02±0.01） and Group C（0.01±0.01） （F=20.38,both P＜0.01）,while no significant difference was shown between Group B and Group C （P＞0.05）.（2） No significant difference was found in the expression of CD4＋CD25＋Foxp3＋Treg cells among different groups （F 0.67,P＞0.05）.（3） The level of IL 10 in Group C was 3.02 ± 3.79,which was significantly lower than that in Group A （10.78±5.44） and Group B （6.90±4.64）（F=4.68,P＜0.01 and 0.05）.The level of IL-10 inGroup B was still lower than that in Group A （P＜0.05）.The levels of TGF-β1 in Group C（8.44± 13.71） and Group B（16.15 ±13.94） were significantly lower than that in Group A（30.23 ± 16.32） （F=5.22,P＜0.01,P＜0.05

关 键 词：免疫球蛋白类 静脉内 胎血 T淋巴细胞 婴儿 早产 

分 类 号：R722.6[医药卫生—儿科]