多花黑麦草胚性悬浮系的建立及原生质体培养  

Establishment of Embryogenic Suspension and Protoplast Culture of Italian Ryegrass

作　　者：葛台明[1] 章荣德[1] 秦发兰 余毓君 谢岳峰 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室

出　　处：《华中农业大学学报》2000年第4期310-312,共3页Journal of Huazhong Agricultural University

基　　金：湖北省教委青年项目资助

摘　　要：以多花黑麦草 (LoliummultiflorumLam .)幼穗为外植体诱导愈伤组织 ,经高有机态氮培养基改造得到易碎型胚性愈伤组织 ,在 1 / 2AA培养基 +1 / 2MSDL培养基中得到了生长迅速、分散的胚性悬浮系。Friable calli of Italian ryegrass ( Lolium multiflorum Lam.) were obtained from young inflorescence culture by subculturing the calli in organic nitrogen rich media and were used to promote the embryogenic suspension cultures. Fast growing, fine dispersed embryogenic suspensions were established in 1/2 AA+1/2 MSDL liquid medium. Protoplasts from these suspensions were cultured. Albinos were regenerated from the protoplast culture.

关 键 词：多花黑麦草 胚性悬浮系 原生质体培养 白花苗 

分 类 号：S543.604.3[农业科学—作物学] S543.603.5