烟草感染马铃薯Y病毒脉坏死株系后PPO活性及其同工酶变化的研究  

Changes of Polyphenoloxidease Activity and Isozyme in Tobacco Infected with Potato Virus Y vein Necrosis Strain

作　　者：文才艺[1] 吴元华[2] 张艳红[3] 

机构地区：[1]襄樊学院化学化工系,襄樊441053 [2]沈阳农业大学植保系,沈阳110161 [3]华东理工大学生物化工学院,上海200237

出　　处：《华中农业大学学报》2000年第4期328-330,共3页Journal of Huazhong Agricultural University

摘　　要：感染马铃薯Y病毒脉坏死株系 (PVYN)后 ,敏感烟草体内的多酚氧化酶 (PPO)活性升高 ,而抗性烟草的PPO活性降低 ,但其活性值均高于敏感烟草健康植株 ;敏感烟草从接种PVYN 后第 8d起出现 2条新的PPO同工酶谱带 ,而且随着病害程度的加重其强度增加 ,抗性烟草则没有新的PPO同工酶谱带出现 .在未接种的情况下 ,抗性烟草体内的PPO活性明显高于敏感烟草 ,其PPO同工酶谱带数比敏感烟草多After being infected with potato virus Y vein strain(PVY\+N), the activity of polyphenoloxidase(PPO) increased in susceptible tobaccos, whereas decreased in resistant ones. However, its absolute value was greater than that in the control plants—the healthy plants of succeptible cultivar. Two new PPO isozyme bands appeared at the eighth day after inoculation, and they gradually enhanced with the development of disease, while there was no new isozyme band showed in resistant tobacco plants. Without inoculation, PPO activity in resistant tobacco plants was obviously greater than that in susceptible ones,and had one more isoxyme band than that in susceptible tobacco plants.\;

关 键 词：烟草 马铃薯Y病毒脉坏死株系 多酚氧化酶 同工酶 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S432.22[农业科学—植物保护]