高效液相色谱法测定萸苁强肾胶囊中多组分的含量  

Determination of ursolic acid, oleanolic acid and tenuifolin in Yucong Qiangshen capsules by HPLC

作　　者：冯晓川[1] 冯金鸽[2] 张蕊[1] 

机构地区：[1]北京积水潭医院,北京100035 [2]首都医科大学附属复兴医院,北京10038

出　　处：《中南药学》2013年第11期849-852,共4页Central South Pharmacy

摘　　要：目的应用高效液相色谱法测定萸苁强肾胶囊（YCQSC）中熊果酸、齐墩果酸和细叶远志皂苷的含量。方法色谱柱：C18色谱柱（4.6 mm×250 mm,5μm）;流速：1.0 mL min-1;熊果酸、齐墩果酸以甲醇-0.1%甲酸溶液（75：25）为流动相,检测波长：210 nm;细叶远志皂苷以甲醇-0.1%磷酸溶液（87：13）为流动相,检测波长：210 nm。结果熊果酸和齐墩果酸分别在0.027 6~0.552 0μg（r=0.999 7）、0.010 3~0.206 0μg（r=0.999 5）与峰面积呈良好的线性关系,平均加样回收率分别为99.3%、97.7%,RSD（n=6）分别为0.82%、0.70%;细叶远志皂苷在0.081 4~1.628μg与峰面积呈良好的线性关系（r=0.999 9）,平均加样回收率为98.4%,RSD=0.78%（n=6）。结论该方法测定结果准确、灵敏、重复性好。Objective To develop an HPLC method to determinate the content of ursolic acid, oleanolic acid and tenuifolin in Yucong Qiangshen capsules（YCQSC）. Methods C 18 column was used as the chromatographic column, and the flow rate was 1.0 mL min- 1. For ursolic acid and oleanolic acid：the mobile phase consisted of methanol0.1% formic acid solution（75 ：25）, and the UV detection wavelength was 210 nm. For tenuifolin： the mobile phase consisted of methanol-0.1% phosphoric acid solution（87 ：13）,and the UV detection wavelength was also 210 nm. Results Good linearity was seen for ursolic acid and oleanolic acid within 0.027 6- 0.552 0 μg（r = 0.999 7）,and 0.010 3- 0.206 0 μg（r = 0.999 5） respectively. The average recovery was 99.3%（RSD = 0.82%） and 97.7%（RSD = 0.70%）. That for tenuifolin was within 0.081 4- 1.628 μg（r = 0.999 9）, and the average recovery was 98.4%, RSD = 0.78%（n = 6）. Conclusion The method is accurate, sensitive and reproducible, which may be used to determinate ursolic acid, oleanolic acid and tenuifolin in RCQSC.

关 键 词：萸苁强肾胶囊 熊果酸 齐墩果酸 细叶远志皂苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]