水稻二化螟内共生菌杀雄菌属和沃尔巴克氏体的检测与分析  

Detection and analysis of symbiotic bacteria,Arsenophonus and Wolbachia,in striped stem borer,Chilo suppressalis(Lepidoptera:Crambidae)

作　　者：柴换娜[1] 吴海燕[1] 杜予州[1] 

机构地区：[1]扬州大学园艺与植物保护学院/应用昆虫研究所,江苏扬州225009

出　　处：《应用生态学报》2013年第12期3517-3522,共6页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展计划项目(2012CB114100)资助

摘　　要：应用杀雄菌属（Arsenophonus）23SrDNA基因和沃尔巴克氏体（Wolbachia）wsp基因的特异引物，通过PCR扩增的方法对我国20个水稻二化螟地理种群感染两类内共生菌的情况进行了检测．结果表明：我国水稻二化螟不同地理种群的Arsenophonus和Wolbachia感染率不一致，哈尔滨、惠水、吉林、南阳和扬州5个地理种群的Arsenophonus感染率为5．0％～50．0％；汉中、南宁和扬州3个地理种群的Wolbachia感染率为25．0％～40．0％，其他地理种群中没有检测到这两种共生菌的存在．水稻二化螟不同地理种群感染的Arsenophonus23SrDNA基因序列完全一致，将该基因株型命名为csArs．但所检测到的Wolbachiawsp基因序列不一致，分别为wChisupl、wChisup5和wChisup6，其中wChisupl属于A群，其他属于B群．这说明水稻二化螟感染的Arsenophonus为同一株型，而感染的Wolbachia株型较为复杂．通过构建系统发育树发现，水稻二化螟体内的Arsenophonus23SrDNA基因和Wolbachiawsp基因与其他物种体内相关序列完全一致或高度相似．In this study, 23 S rDNA from Arsenophonus and wsp gene from Wolbachia were amplified by PCR method using specific primers to study their symbiosis in Chilo suppressalis from 20 locations of China. The results indicated that Arsenophonus and Wolbachia infection rates differed widely in C. suppressalis populations. The infection rates of Arsenophonus ranged from 5.0% to 50.0% in five geographical populations from Harbin, Huishui, Jilin, Nanyang, and Yangzhou. Wolbachia ranged from 25.0% to 40.0% in three geographical populations from Hanzhong, Nanning, and Yangzhou. Their symbiosis was not observed in C. suppressalis of other locations. The sequences of 23 S rDNA gene which named csArs were exactly the same in the five populations. However, the se- quences of wsp from the three strains of Wolbachia showed wChisupl belonged to supergroup A, wChisup5 and wChisup6 belonged to supergroup B. The results showed the strains of Arsenophonus from C. suppressalis of the five different locations were identical, whereas the strains of Wolbachia were phylogenetically diverse. The phylogenetic analysis indicated that 23S rDNA and wsp sequences found in C. suppressalis were exactly the sequences in other species. same with or very closely related to relevant

关 键 词：水稻二化螟 内共生菌 ARSENOPHONUS WOLBACHIA 系统发育分析 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]