褪黑素对成年大鼠海马神经元络丝蛋白表达的影响  

Influence of Melatonin on Reelin Expression in Hippocampus Neurons of Adult Rats

作　　者：王澜[1] 王淑莲[1] 

机构地区：[1]娄底市中心医院新生儿科,湖南娄底417000

出　　处：《贵阳医学院学报》2013年第6期629-631,共3页Journal of Guiyang Medical College

摘　　要：目的:探讨褪黑素对络丝蛋白(reelin)表达的影响。方法:将SD大鼠分为生理盐水对照组和褪黑素组,两组大鼠腹腔分别给予生理盐水或褪黑素后,用Western blot、免疫组织化学以及实时定量PCR的方法检测海马组织中reelin表达。结果:褪黑素组大鼠海马神经细胞胞膜以及细胞外基质中reelin表达与对照组相比明显增加(P<0.05),reelin mRNA的表达量增加(P<0.05),Western blot结果显示给予褪黑素后,大鼠海马reelin蛋白表达增加(P<0.05)。结论:褪黑素可能通过增加reelin mRNA的量促进reelin蛋白的表达。Objective： To investigate influence of melatonin on reelin protein expression. Methods： Twenty SD rats were randomly divided into melatonin treatment group （group reel）, and normal saline control group （group con） , and were given melatonin and saline respectively via abdominal cavity. Reelin expression in hippocampus was detected by using Western blot, immunohistochemical method and real time PCR. Results： The expression of reelin in cell membrane and extracellular matrix of group mel was significantly higher than those in group con. The amount of reelin mRNA of group mel was 2.5 times of that of group con, and reelin protein increased by 1.5 times after melatonin treat- ment. Conclusion： Melatonin can increase the quantity of reelin by promoting mRNA expression in the cell membrane and extracellular matrix.

关 键 词：褪黑激素 海马 神经元 络丝蛋白 大鼠 SPRAGUE-DAWLEY 

分 类 号：R338.24[医药卫生—人体生理学]