海螵蛸一骨形态发生蛋白复合材料对骨缺损大鼠成骨及再血管化的影响  

Effects of cuttlefish bone-bone morphogenetic protein composite material on osteogenesis and revas- cularization of bone defect in rats

作　　者：刘源[1] 于江[2] 白洁[2] 顾劲松[2] 蔡斌[1] 周霞[3] 

机构地区：[1]江苏省连云港市第二人民医院烧伤整形科,222000 [2]大连医科大学美容医学院 [3]海军大连舰艇学院

出　　处：《中华烧伤杂志》2013年第6期548-553,共6页Chinese Journal of Burns

摘　　要：目的研究海螵蛸一骨肜态发生蟹白（BMP）复合材料对骨缺损大鼠成骨及再血管化的影响、方法将海螺蛸去外壳，制成圆柱体（直径约5mm，高约2toni），浸泡于重组入骨形态发生蛋白2溶液flI制作海螵蛸-BMP复合材料。将30只SD大鼠制成颅骨缺损模剂，按照随机数字表法分成海螵蛸一BMP纰与单纯海螵蛸组，每组15只，分别植入相应材料修复颅骨缺损。移植术后4、6、8蚓，2组各取5只大鼠．分批放血处死同时行墨汁灌注。ld后取出全部植入物和周围部分颅骨同时进行植入区大体观察，制作切片行Hf￡及Masson染色，通过组织病理学技术、图像采集系统，观察、分析做血锊面积卡¨新生骨面积。对数据行两因素析因设计方差分析和LSD检验，并对海螵蛸一BMP组入鼠做I血管面积和新生骨面积的相关性行Pearson相关分析。结果（1）植入区大体观察可见植入物被纤维结缔组织包膜包裹，移植术后4周包膜质地柔软、较厚；移植术后6、8周包膜质韧、薄，但较致密。植人物移植术后4同时转为胶冻样物质，移植术后6、8同时与软骨组织类似。（2）组织学观察显示．移植术后4周，2组大鼠植入物结构均遭到破坏，并可见中等数量的炎性细胞浸润；海螵蛸一BMP组初级骨小梁、微血管数量明显多于单纯海螵蛸组，破骨细胞数鼠则少于单纯海螵蛸组。移植术后6周，2组大鼠植入物炎性细胞明显减少，海螵蛸进一步降解，新生微血管更加丰富，植人物周边均彤成明显的成熟骨小梁，但海螵蛸一BMP组在降解后的海螵蛸一BMP中也出现了成熟骨小梁。移植术后8周，2组大鼠植入物炎症反应消失，成熟’目r，J、梁显著增多，海螵蛸的网格状结构基本被破坏，单纯海螵蛸组的成熟骨小梁仍分布在海螵蛸周边；而海螺蛸一BMP组中，海螵蛸．BMP中央、周围均可成熟骨小梁，徽血管数�Objective To study the effects of cuttlefish bone-bone morphogenetie protein （BMP） composite material on ostecgenesis and revast＇ularization of bone defect in rats. Methods The cuttlefish bone was formed into cylinder with the diameter of about 5 mm and height of ahout 2 mm after the shell was remt^ved, and then it was soaked in the recombinant human BMP 2 to make a cuttlefish bone-BMP （CBB） composite material. Thirty SD ruts, with a defect of skull in every rat, were divided into Ihe CBB and pure c＇utllefish hone （PCI：~） groups according to the random number table, with 15 ruts in each group. The rats in the group CBB and group PCB were transplanted with the corresponding material to repair the skull defect. At post transplantalion week （ PTW） 4, 6, and 8, 5 rats from every group were sacrificed by exsanguina- ticm, and ink perfilsion was perh）rmed. One dav later, all the transplants and part of the skull surrounding the defect were harvested, and general observation was conducted at the same time. The specimens were par- affin sectioned for HE staining and Masson staining. The area of microvessel and the area of newborn bone were observed and analyzed through histopathological techniques and image collection system. Data were pro- cessed with the analysis of variance of factorial design and LSD test. The correlation between the area of mi- crovessel and the area of newborn bone of the group CBB was analyzed with Pearson correlation analysis. Results （ 1 ） The general observation of the transplant region showed that the transplants were encapsulated by a capsule of fibrous connective tissue. The texture of capsule was soft and relatively thick at PTW 4. The texture was tenacious and thin, but rather compact at PTW 6 and 8. The transplants became gelatinous at PTW 4, and similar to the cartilage tissue at PTW 6 and 8. （2） Histological observation showed that the structure of the transplants in two groups was damaged at PTW 4. A moderate quantity of inflammatory cell infiltration co

关 键 词：骨形态发生蛋白质类 海螵蛸 成骨 再血管化 

分 类 号：R681[医药卫生—骨科学]