鸡传染性支气管炎病毒变异株的分离及分子特征研究  

Isolation and molecular characterization of an avian infectious bronchitis virus variant

作　　者：边葶苈[1] 朱林文思 雷静[1] 任景乐[2] 李烨[1] 周继勇[1] 廖敏[1] 

机构地区：[1]浙江大学农业部动物病毒学重点实验室,浙江杭州310058 [2]青岛市畜牧兽医研究所,山东青岛266100

出　　处：《中国预防兽医学报》2013年第12期960-963,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：现代农业产业技术体系专项经费项目(CARS-41-11);教育部新世纪人才项目(NCET-09-0691)

摘　　要：为了解近年来鸡传染性支气管炎病毒（IBv）分子生物学特征及其变异情况，本研究以最近从山东潍坊某鸡场产蛋鸡卵巢中分离的一株IBV WF2011为研究对象，进行基因2～基因6的基因组测序及分析。结果表明分离株3’端基因组具有典型的IBV基因编码特征5’．S．3a．3b．E．M．5a．5b．N．3’，S蛋白裂解位点为ⅫRRSRR，，与美国株Gray、Holte、JMK、Conn46和韩国株SNU8067的s蛋白裂解位点相同。同源性比较和系统进化分析显示，WF2011分离株与常见的参考病毒株的同源性普遍较低，s1基因的同源性在49．7％～86．0％之间，并且与LX4、SAlBK和Mass类型的病毒株存在较大的遗传距离。WF2011是一株变异程度比较大的IBV变异株。To investigate the molecular characteristics and variation of newly isolated infectious bronchitis virus （IBV）, the gene2 to gene6 genomic sequences of IBV WF2011 isolated from ovarian tissues of laying hens in Weifang of Shandong were sequenced. The sequence analysis showed that the C terminal sequence of WF2011 had the typical 5＇-S-3a-3b-E-M-5a-5b-N-3＇ gene order and the typical avian coronavirus cleavage site of ~RRSRR~ in spike protein, as American strains of Gray, Holte, JMK, Conn46 and South Korean SNU8067 strain. Homology analysis and phylogenetic analysis showed that WF2011 had low identities with IBV reference strains. The identities of WF2011 S1 gene with those of other reference strains range from 61.8% to 86.0%. There is relatively far genetic distance between WF2011 and LX4, SAIBK, Mass-like strains. Therefore, WF2011 might be a new variant IBV isolate.

关 键 词：鸡传染性支气管炎病毒 变异株 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]