H3N2亚型犬流感灭活疫苗的免疫效果评价  

Evaluation of the protective efficacy and immunogenicity of inactivated canine influenza virus(H3N2) vaccine

作　　者：赵明喜[1] 赖芳芳[1] 贾坤[1] 李华涛[1] 远立国[1] 李守军[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国预防兽医学报》2013年第12期993-996,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30972233);广东省科技计划项目(2008A060202017;2009B060300024);广东省自然科学基金重点项目(8251064201000008);教育部博士点基金(20094404110017);重要病毒跨种间感染与传播致病的分子机制研究(2011CB504700-1)

摘　　要：为研制H3N2亚型犬流感病毒灭活疫苗,本实验通过鸡胚传代的方法培育出一株高繁殖能力的鸡胚适应株CGD1株。分别应用白油和氢氧化铝胶佐剂制备两种灭活疫苗,通过监测疫苗免疫后BALB/c小鼠和比格犬的抗体水平以及比格犬的攻毒保护试验,评价两种疫苗的免疫效果。结果表明,两种佐剂疫苗均能够诱导小鼠和犬产生较强的免疫应答反应,二免后14 d,免疫犬抗体水平均高于1∶40;攻毒试验中,疫苗免疫可以显著减轻病毒感染犬临床症状,明显缩短病毒的排毒期,特异性抗体水平显著提高。本研究研制了两种佐剂H3N2亚型犬流感灭活疫苗,为犬流感的防制提供保障。To evaluate the immunogenicity and protective efficacy of the inactivated H3N2 subtype canine influenza virus （CIV） vaccine emulsified in mineral-oil or aluminum hydroxide adjuvant, the BALB/c mice and Beagle pups were immunized with the vaccines, respectively, and the pups was challenged with avian-origin H3N2 subtype virulent CIV post vaccination （PI）. The results showed that the vaccinated mice and pups developed high titers of hemagglutination inhibition antibody PI. In addition, the vaccinated pups did not show any clinical signs and the duration of virus shedding was significantly shorter than that in the control group after challenge. These results indicated that both the two adjuvant vaccines were able to induce strong immune response and provide protection against the H3N2 subtype CIV challenge in dogs. However, the mineral-oil adjuvant vaccine was superior to the aluminum hydroxide adjuvant vaccine.

关 键 词：犬流感 H3N2亚型 灭活疫苗 佐剂 

分 类 号：S852.65[农业科学—基础兽医学]