猪肺炎支原体P97R1抗原免疫刺激复合物的制备及对活疫苗免疫刺激能力的增强作用研究  

Preparation of Mycoplasma hyopneumonia immunostimulating complex of P97R1 and the enhancement of ISCOM-P97R1 to the immunostimulating effect on the live vaccine

作　　者：韦艳娜[1] 熊祺琰[1] 董璐[1] 冯志新[1] 邵国青[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《中国预防兽医学报》2013年第12期1016-1019,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省农业自主创新基金项目[cx(12)5047]

摘　　要：为增强肌肉注射免疫条件下猪肺炎支原体(Mhp)活疫苗的免疫刺激能力,并补充其缺乏的针对重要黏附因子P97R1的应答,本实验采用透析法制备含有P97R1的免疫刺激复合物(ISCOM-P97R1),并将ISCOMP97R1作为活疫苗的佐剂,通过小鼠模型进行免疫学评价。结果表明,电镜下观察所制备的ISCOM-P97R1具有ISCOM的典型蜂窝状结构,直径为30 nm^40 nm,P97R1蛋白的包封率可达85.6%。免疫后7 d即可在小鼠血清中检测到高水平的特异性P97R1抗体,并且其针对Mhp全菌蛋白的抗体也显著高于无佐剂的活疫苗对照组。结果表明,ISCOM-P97R1对Mhp活疫苗免疫刺激能力具有显著的增强作用,同时可以有效诱导产生针对P97R1的免疫应答,进一步增强了疫苗的保护能力。To investigate the enhancement of immunostimulating complex containing P97R1 antigen （ISCOM-P97R1） to the immunostimulating effect of the Mycoplasma hyopneumoniae live vaccine via intramuscular injection, as well as its effect to supplement the immune response to the important adhesions P97R1, ISCOM-P97R1 was prepared by dialysis method, which was subsequently used as an adjuvant for the live vaccine. The immunologic effect was evaluated in the mice model. The prepared ISCOM-P97R1, with a size of 30 nm to 40 nm, showed a cage-like structure under electron microscope. The entrapment rate of protein prepared was 85.6%. In addition, One week after the inoculation, the high levels of antibody against P97R1 and whole M.hyopneumoniae proteins could be detected in the serum of the mice immunized with the ISCOM-P97R1 adjuvanted live vaccine, which was significantly higher than that in the mouse group immunized with the live vaccine without adjuvant. In conclusion, ISCOM-P97R1 showed a significant enhancement to stimulate immune response on the live vaccine, and induced the immune response to P97R1 effectively which further enhanced the protection ability of the vaccine.

关 键 词：免疫刺激复合物 P97R1 猪支原体肺炎 活疫苗 

分 类 号：S852.62[农业科学—基础兽医学]