类风湿关节炎患者B细胞凋亡与白细胞介素-17相关性研究  

Associations of apoptosis with B cell and expression of interleukin-17 in patients with rheumatoid arthritis

作　　者：杨晋[1] 胡文霞[1] 赵绍林[1] 杨新玲[1] 吴惠毅[1] 

机构地区：[1]江苏省连云港市第一人民医院临床实验中心,222002

出　　处：《中华风湿病学杂志》2013年第12期815-817,共3页Chinese Journal of Rheumatology

摘　　要：目的探讨类风湿关节炎（RA）患者外周血中B细胞凋亡与血清中白细胞介素（IL）-17表达相关性及临床意义。方法流式细胞术检测80例初诊RA患者及80名健康对照外周血B细胞凋亡比例。选择20例RA患者和20名健康对照，免疫磁珠法分选外周血中B细胞；采用蛋白印迹法检测外周血B细胞凋亡相关蛋白B细胞淋巴瘤．2（Bcl-2）、Caspase-3蛋白水平表达；酶联免疫吸附试验（ELISA）法检测RA患者血清中IL-17表达。采用t检验及线性回归分析进行统计学处理。结果B细胞在RA患者及对照者外周血中凋亡比例分别为：（14±6）％、（24±9）％，RA患者外周血B细胞凋亡比例低于对照组（t=2．737，P=0．021）。RA患者外周血B细胞抗凋亡基因Bcl-2蛋白表达水平高于对照（分别为26±10，12±6，P〈0．01），凋亡基因Caspase-3蛋白表达水平低于对照（分别为16±7，31±12，P〈0．01）。RA患者血清中IL-17表达高于对照组[分别为（69±19），（27±10）pg／ml，t=4．631，P=0．014]，RA患者血清IL-17升高与外周血B细胞凋亡呈负相关（r-0．36，P〈0．01）。结论RA外周血B细胞凋亡相关蛋白Caspase-3表达降低，抗凋亡蛋白Bcl-2表达升高，RA外周血B细胞凋亡途径受到抑制。RA血清IL-17表达与外周血B细胞凋亡抑制显著相关，IL-17可能抑制B细胞凋亡。Objective To investigate the relation between the apotosis of B cells in the peripheral blood （PB） and the expression of interleukin （IL）-17 in patients with rheumatoid arthritis （RA）. Methods The proportions of apoptosis of B cells in the PB of 80 patients with RA and 80 healthy controls were measured by flow cytometry. B cells in the PB of 20 RA and 20 healthy individuals were isolated by MACS and Western blotting was used to detect the Bcl-2 and Caspase-3 protein levels. IL-17 levels were detected by enzyme-linked immunosorbent assay （ELISA）. T-test and linear regression were used to analyze the data. Results The proportions of apoptosis of B cells in the PB of patients with RA and healthy controls were （14±6）% and （24±9）% respectively. The rate of apoptosis of B cells in patients with RA was significantly less than healthy controls （t=2.737, P=0.021 ）. The Bcl-2 protein level of B cells in the PB of patients with RA group was significantly higher than that of control group （26±10, 12±6, P〈0.01 ）. Conversely, the Caspase-3 protein level of B cells in the PB of patients with RA group was significantly lower than that of the control group （16±7, 31±12, P〈0.01）. ELISA detected elevated level of serum IL-17 in the patients with RA as compared with controls [（69±19）, （27±10） pg/ml, t=4.631, P=0.014]. There was a negative correlation between the level of IL-17 and apoptosis of B cells in patients with RA （r=0.36, P〈0.01 ）. Conclusion The elevated bcl-2 and reduced caspase-3 of B cells in patients with RA further proves there is abnormal apoptosis of B cells in RA patients. There is negative correlation between the expression of IL-17 and apoptosis of B cells in patients with RA and IL-17 can inhibit B cell apoptosis.

关 键 词：关节炎 类风湿 B细胞 细胞凋亡 白细胞介素-17 

分 类 号：R593.22[医药卫生—内科学]