血浆miRNA-124和miRNA-765作为重型颅脑创伤患者生物标记物的研究  

Plasma miRNA-124 and miRNA-765 as biomarkers for severe traumatic brain injury

作　　者：李爱林[1] 王琼[1] 只达石[1] 

机构地区：[1]天津市环湖医院颅脑创伤中心,300061

出　　处：《中华神经医学杂志》2013年第12期1283-1285,共3页Chinese Journal of Neuromedicine

摘　　要：目的研究重型颅脑创伤（sTBI）后血浆中miR-124和miR-765表达水平的变化。方法选择天津市环湖医院颅脑创伤中心自2009年1月至2010年3月收治的30例急性sTBI患者，设为实验组，另选24例同期行健康体检的成年人为对照组，RT-qPCR检测颅脑创伤患者不同时间段血浆和伤后脑创伤组织中miR-124和miR-765的表达水平，受试者工作特征曲线（ROC）分析这两种miRNA可否作为判断sTBI的依据。结果miR-124水平在伤后6h显著升高，并且在伤后12h、24h继续增高；miR-765在12h达到高峰，与对照组之间比较差异有统计学意义q=2．411，P=0．001）。sTBI患者脑挫裂伤组织比对照组组织中miR-124和miR-765表达水平显著升高，差异有统计学意义（t=2．686，P=0．001；t=2．673，P=0．000）。ROC表明miR-124和miR-765是辅助诊断sTBI很好的血浆标志物（AUC值分别为0．98和0．79）。结论sTBI患者血浆miR-124和miR-765的表达显著改变，可以作为sTBI严重程度的标志物。Objective To investigate the changes of plasma miRNA-124 and miRNA-765 levels in patients with severe traumatic brain injury （sTBI）. Methods Thirty sTBI patients, admitted to our hospital from January 2009 to March 2010, and 24 healthy volunteers were included in the study. Quantitative RT-PCR was employed to determine the levels of miRNA -124 and miRNA -765 in the plasma and traumatic brain tissues at different time points after injury; receiver operating characteristic curve （ROC） was used to explore the potential of miRNA -124 and miRNA -765 as diagnostic TBI biomarkers. Results MiRNA -124 level dominantly increased 6 h after TBI and continued to rise 12 and 24 h, while miRNA -765 reached its peak level at 12 h after TBI in patients with sTBI, enjoying significant differences as compared with those in the matched healthy volunteers（t=2.41, P=0.001）. miR- 124 level （t=2.686, P=-0.001） and miR-765 level （t=2.673, P=0.000） in the contusion and laceration tissues after sTBI predominantly increased as compared with those in the normal brain tissues surrounding to the contusion and laceration tissues, with significant difference. ROC curve analysis indicated that miR-124 and miR-765 were good diagnostic markers of sTBI （area under the curve： 0.98 and 0.79）. Conclusion Plasma-derived miR-124 and miR-765 change significantly, which could be employed as diagnostic markers for sTBI.

关 键 词：重型颅脑创伤 血浆 MIRNA 

分 类 号：R651.1[医药卫生—外科学]