非同源脑脊液诱导非同源间充质干细胞分化为神经干细胞的对比研究  

A comparative study on Inducing Non-homologous Mesenchymal Stem Cells to Differentiate into Neural Stem Cells Using Non-homologous Cerebrospinal Fluid

作　　者：任超[1,2] 刘小云[3] 万美荣[3] 耿德勤[4] 葛巍[4] 李金梅[4] 张伟伟[4] 

机构地区：[1]徐州医学院神经病学教研室,徐州221002 [2]山东省泰安市中医医院脑病中心,泰安271001 [3]徐州医学院附属医院中心实验室,徐州221002 [4]徐州医学院附属医院神经内科,徐州221002

出　　处：《生物医学工程学杂志》2013年第6期1290-1297,共8页Journal of Biomedical Engineering

摘　　要：寻找一种符合患者治疗需求的干细胞获取模式,从而进一步推动神经干细胞广泛应用于临床。本实验采用志愿者、患者脑脊液体外诱导异体脐血源间充质干细胞(UMSCs)和患者骨髓源间充质干细胞(BMSCs)分化为神经干细胞,并采用形态学、流式细胞分析、免疫组化、荧光免疫组化法等方法对诱导后的细胞进行签定;同时,记录各诱导组获得临床可用神经干细胞所需的时间及细胞数计数。结果证实各组均可使间充质干细胞(MSCs)定向分化为神经干细胞,所获得的细胞数亦可满足临床需要,但在所需的天数及具体获得细胞数量上各组间有一定差异,其中以患者BMSCs用自愿者脑脊液诱导组所获得神经干细胞计数最高,且其所需时间最短。In order to set up a base for stem cells to be widely used in clinical medicine, we tried to optimize, in this study, the technique that induces human mesenchymal stem cells （hMSCs） to differentiate into neural stem ceils by using cerebrospinal fluid （CSF） from the different groups. After the induction, presence of neural stem cells was confirmed with microscope observation, flow cytometry analysis, immunohistochemistry and fluorescent immunohis tochemistry. At the same time, we also compared and analysed the data of the number of stem ceils when it totally met the requirements for clinical treatment and the days required. At last, we confirmed that hMSCs could be in duced to differentiate into neural stem cells, and that the number of cells totally met the requirements for clinical treatment. But there were some differences both in the number of cells and the days required. Among the groups, the group that marrow mesenchymal stem cells from patient＇s own induced by CSF from healthy volunteers used the shortest time and the quantity of the cells was significantly higher than those of the others.

关 键 词：骨髓 脐血 间充质干细胞 脑脊液 诱导 神经干细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]