姜黄素通过降低一氧化氮合酶水平抑制宫颈癌HeLa细胞的侵袭和转移  

Curcumin inhibits HeLa cell invasion and migration by decreasing inducible nitric oxide synthase

作　　者：李牧[1] 王丽[1] 刘海莉[1] 苏宝山[2] 刘变利[1] 林温静[1] 李昭荣[1] 常黎华[3] 

机构地区：[1]西安交通大学医学院第二附属医院妇产科,陕西西安710004 [2]西安交通大学医学院第二附属医院病理科,陕西西安710004 [3]西安交通大学医学院第二附属医院预防与保健科,陕西西安710004

出　　处：《南方医科大学学报》2013年第12期1752-1756,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30472170)~~

摘　　要：目的探讨姜黄素抗宫颈癌HeLa细胞侵袭转移的作用及其可能机制。方法分别以0、10、25、50、100、150和200μmol/L浓度的姜黄素干预宫颈癌HeLa细胞。24 h后,采用MTT法观察其对HeLa细胞增殖的抑制作用,TUNEL法染色观察其对HeLa细胞凋亡的诱导作用。应用Transwell小室检测姜黄素对HeLa细胞侵袭转移能力的抑制作用,Western-blot检测其对金属基质蛋白酶-9(MMP-9)以及E-钙粘素(E-cad)表达水平的影响。通过RT-PCR及Western-blot检测姜黄素对诱生型一氧化氮合酶(iNOS)表达水平的影响;采用Griess法观察姜黄素对HeLa细胞生成一氧化氮(NO)能力的影响。结果姜黄素可通过诱导凋亡抑制HeLa细胞的增殖,且呈现浓度依赖性;姜黄素可显著降低HeLa细胞的侵袭能力并能够降低MMP-9的表达水平,同时升高E-cad的表达水平;姜黄素可显著降低HeLa细胞内iNOS的表达水平,并降低NO的合成。结论姜黄素可能通过降低iNOS的表达水平抑制HeLa细胞的侵袭、转移能力。Objective To investigate the inhibitory effects of curcumin against HeLa cell invasion and migration and explore the underlying mechanisms. Methods HeLa cells were exposed to curcumin treatment at the concentrations of 0, 10, 25, 50, 100, 150 and 200 μmol/L for 24 h. MTT and TUNEL assays were used to assess the cell proliferation inhibition and apoptosis, respectively. Transwell assay was used to evaluate the invasiveness and migration of the treated cells, and RT-PCR and Western blotting were employed to detect the changes in the expression of inducible nitric oxide synthase （iNOS）, and MMP-9 and E-cad, the 2 markers of cell invasion and migration, were detected by Western blotting. The capacity of NO production in HeLa cells was measured by Griess method. Results Curcumin inhibited the proliferation of HeLa cells by inducing cell apoptosis in a concentration-dependent manner. Curcumin inhibited the invasion and migration of HeLa cells by increasing E-cad expression and decreasing MMP-9 expression, and also decreased the expression level of iNOS and NO production in the cells. Conclusions Curcumin inhibits the invasion and migration of HeLa cells by decreasing the expression of iNOS.

关 键 词：姜黄素 宫颈癌 侵袭 转移 HELA细胞 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]