汉防己甲素对于膈疝模型大鼠胎肺中血管内皮生长因子表达的影响  

The effect of tetrandrine on the expression of VEGF in the lung of diaphragmatic hernia model rat

作　　者：陈中献[1] 徐畅[1] 刘文英[2] 

机构地区：[1]四川大学华西医院小儿外科,成都610041 [2]四川省人民医院儿童医学中心

出　　处：《中华小儿外科杂志》2013年第12期933-937,共5页Chinese Journal of Pediatric Surgery

摘　　要：目的研究血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）在大鼠膈疝模型胎肺中的表达特点及规律，以及汉防己甲素（Tet）干预后的变化。方法20只健康怀孕的SD大鼠雌鼠在孕9．5d时用随机数字表法随机分为3组：即正常对照组（C组）6只；膈疝组（D组）7只；膈疝汉防己甲素组（DT组）7只。D组和DT组灌胃给予除草醚（Nitrofen125mg／只，溶于2ml橄榄油中），C组接受等量的橄榄油，DT组于孕11--13d每天给予30mg·kg^-1·d^-1Tet灌胃，C组和D组仅给予生理盐水。分别于第16d、18d和21d麻醉下剖宫取胎鼠双肺，应用组织学观察及原位杂交方法研究VEGF在膈疝模型不同时段胎鼠肺的表达特点及规律，及应用汉防己甲素后VEGF在膈疝模型不同时段胎鼠肺的表达特点及变化规律。结果本组实验中D组18d（D18）和21d（D21）的膈疝发生率为85．2％和DT组18d（DT18）和21d（DT21）的膈疝发生率为76．7％，差异无统计学意义（P〉0．05）。C组16d（C16）、18d（C18）和21d胎龄胎鼠（C21）胎肺VEGFmRNA分光光密度（integralopticaldensity，IOD）分别为7493．4±3167．9、4024．7±2204．9和8697．4±1466．8。D组16d（D16）、18d（D18）和21d胎龄胎鼠（D21）ga肺VEGFmRNAIOD分别为15269．2±5307．5、5670．5±1588．5和8061．3±2245．7。DT组16d（DT16）、18d（DT18）和21d胎龄胎鼠（DT21）胎肺VEGFmRNAI（）D分别为10742．8±4803．5、5626．4±3231．3和11687．7±11628．7。D18与D16和D21与D16的IOD比较差异有统计学意义（P〈0．05）。VEGFmRNA阳性表达的分布特点：C16、D16与DT16的胎肺VEGFmRNA阳性表达位于呼吸道内皮细胞胞浆，尤以远端呼吸道出芽区域更为显著。C18、D18、DT18、C21、D21与DT21的胎肺支气管壁黏膜和血管壁内皮细胞、血管壁肌层可见VEGFmRNA的阳性表达。C21胎肺VEGFmRNA阳性表达部位主要位于肺泡壁和肺问质，呈网状，而D21胎肺肺泡壁Objective To study the expression of vascular endothelial growth factor （VEGF） in the fetal lung of diaphragmatic hernia model rat and the effect of tetrandrine （TET） on its expression. Methods Twenty pregnant Sprague-Dawlay rats were randomly divided into 3 groups： control group （group C） ,nitrofen group （group D） and tetrandrine group （group DT）. The rats in group D and DT were administrated with 125 mg of nitrofen （dissolved in 2 ml of olive oil） by gastric gavage on day 9. 5 of gestation,whereas those in group C only received olive oil of same volume. The rats in group DT were administrated with Tet （30 mg/kg） once a day from gestational day 11 to 13, while their counterparts in group C and D were treated with saline. On day 16, 18 and 21 of laestation, fetal luneswere obtained. The histological observation and the in situ hybridization were used to study the ex- pression of VEGF in the lungs. Results Incidence of CDH for D18 and D21 was 85.2% and 76. 7% for DT18 and DT21. No significant difference of CDH incidence was seen between the two groups （P）0. 05）. IOD of VEGF mRNA in the lungs on d16, dlS, and d21 were 7493.4 ± 3167. 9, 4024. 7 ±2204. 9,8697. 4 ± 1466. 8, respectively in group C and 1 5269. 2 ± 53/）7. 5,5670. 5 ± 1588. 5, 8061.3 ±2245.7, respectively in group D and 1 /）742. 8 ± 4803.5, 5626. 4 ±3231.3, 1 1687. 7 ± 1 1628.7, respectively in group DT. There was significant difference （P%/）. 05） between IOD-DI8 and IOD-D16 and between IOD-D21 and IOD-D16. VEGF mRNA was expressed in the cytoplasm of respiratory endothelial cell, especially in the distal respiratory budding area in C16,D16 and DTI6 fetal lungs. VEGF mRNA expression was also observed in the mucosa of bronchial wall and in the endothe- lial ceils and museularis of hemal wall in Cl8, D18, DT18,C21 ,D21 and DT21. VEGF mRNA expres- sion was mainly found in alveolar walls and interstitial lung in mesh structure in C2l, but not found in D21. The VEGF mRNA expression in DT21 were mainly foun

关 键 词：疝 横膈 血管内皮生长因子类 汉防己甲素 

分 类 号：R655.6[医药卫生—外科学]