野牦牛成纤维细胞分离培养与部分生物学特性观察  

Isolation and Culture of Fibroblast Cells from Bos mutus and Analysis on Some Biological Characteristics

作　　者：郭志林[1,2] 杨永梅[3] 赵明德[1] 于军[1] 王伟[1] 庞礴[2] 郭松长[2] 赵新全[2] 

机构地区：[1]青海民族大学化学与生命科学学院,西宁810007 [2]中国科学院西北高原生物研究所,西宁810008 [3]青海民族大学公共管理学院,西宁810007

出　　处：《中国细胞生物学学报》2013年第12期1748-1752,共5页Chinese Journal of Cell Biology

基　　金：教育部科学技术研究重点项目(批准号:211192);青海民族大学高层次人才科研项目(批准号:2012G002);财政部战略生物资源科技支撑专项(批准号:KSCXZ-YW-Z-0951)资助的课题~~

摘　　要：野牦牛是青藏高原珍稀牛种,国家一级保护动物。为了保存野牦牛遗传资源,采用组织块法建立了3株野牦牛成纤维细胞株。体外培养的野牦牛细胞呈现典型的成纤维细胞形态,增殖能力强,测定的细胞群体倍增时间为38.47 h,平台期密度为2.08×106/mL。经免疫荧光染色,细胞表达FGFR5,经单克隆培养建立了FGFR5阳性细胞株。F7细胞染色体核型分析表明,二倍体正常核型率为84.33%,核型2n=60,常染色体均为近端着丝粒染色体,X、Y染色体为近端着丝粒染色体。所建立的野牦牛体细胞株为开展野牦牛克隆研究提供了材料。Bos mutus is the rare bovine species under first-grade protection and is native to the Qinghai- Tibet Plateau. In order to preserve Bos mutus genetic resource, three fibroblast cell strains from Bos mutus were established by tissue culture. Bos mutus cells in vitro culture appeard typical morphology of fibroblast cell and had strong hyperplasia ability. Population doubling time of the cells was 38.47 h, and plateau phase density of the cells was 2.08×10^6/mL. FGFR5 expression in cells was confirmed by immunofluorescence staining. Cell strains with FGFR5 positive expression were established by monoclonal cell culture. Chromosome karyotypes analysis to F7 cells showed that the rate of normal diploidy （2n=60） was 84.33%. Moreover, all autosomes were acrocentric chromosomes, and X as well as Y chromosome belonged to acrocentric chromosomes. Bos mutus cell strains eslablished in the study offered the material for the Bos mutus cloning research.

关 键 词：野牦牛 细胞培养 成纤维细胞 免疫荧光染色 

分 类 号：S865[农业科学—野生动物驯养]