表皮生长因子纳米微粒对糖尿病大鼠皮肤创面愈合的影响  

Effect of epidermal growth factor nanoparticles promoting wound healing of diabetic rats

作　　者：徐俊[1] 王鹏华[1] 褚月颉[1] 丁敏[1] 田应芳[1] 冯书红[1] 于德民[1] 董晓庆[2] 常津[2] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所卫生部激素和发育重点实验室糖尿病足科,300070 [2]天津大学材料学院纳米生物技术研究所

出　　处：《中华糖尿病杂志》2013年第11期674-677,共4页CHINESE JOURNAL OF DIABETES MELLITUS

摘　　要：目的 观察表皮生长因子（EGF）纳米微粒对糖尿病大鼠皮肤创面的愈合作用,探讨EGF纳米微粒促进愈合的机制.方法 制备EGF纳米微粒,测定EGF纳米微粒载药率、包封率和释药行为.用打孔器制备糖尿病大鼠皮肤创面模型.分为4组：EGF纳米组（给予含1μg/d EGF的纳米微粒）,EGF组（给予1μg/d EGF）,空微粒组（给予不含EGF纳米微粒）,磷酸盐缓冲液（PBS）对照组,于3、7、14、21 d照相并取材,计算创面愈合率并比较创面病理学变化及EGF受体阳性细胞面积.结果 EGF纳米微粒平均粒径为193.5 nm,载药率为0.02％,包封率为85％.药物释放达24h.EGF纳米组创面愈合比EGF组快[14 d,（93.8±1.8）％比（89.3±2.3）％,P＜0.05;21 d（96.6±1.6）％比（92.1±4.3）％,P＜0.05].EGF纳米组创面内EGF受体阳性细胞面积高于EGF组[7d,（49.4±6.5）％比（35.1±2.8）％,P ＜0.05;14 d,（80.2±3.8）％比（73.4±1.7）％,P＜0.05].结论 EGF纳米微粒上调创面内细胞EGF受体表达,加速创面的愈合,效果优于EGF.Objective To investigate the effect and mechanism of epidermal growth factor（EGF） nanoparticles on the wounds of diabetic rats.Methods （1） EGF nanoparticles were prepared by the modified multiple emulsion way with poly lactic-co-glycolic acid （PLGA） as carrier.Drug-loaded rate,encapsulation ratio and release of EGF nanoparticles were measured.（2） Diabetic rats model were made with streptozocin （STZ） through vena caudalis.Skin wound model of diabetic rats were prepared by hole puncher and were divided into 4 groups：EGF nanoparticles group （containing 1 μg/d EGF nanoparticles）,EGF group（1 μg/d EGF）,empty nanometer particle group（without EGF but the quality was equivalent to the EGF nanoparticles/d） and PBS control group.（3） Photographs and sampling at 3,7,14,21 days were taken and wound healing rate were calculated.The positive cells area of epidermal growth factor receptor were compared by immunohistochemistry.Results （1） The average particle diameter of EGF nanoparticles was 193.5 nn.The drug loading ratio was 0.02％ and the encapsulation ratio was 85％.The group of EGF nanoparticles released up to 24 h.（2） Healing of wound of EGF nanoparticles group was fast than that of EGF [14d：（93.8±1.8）％ vs（89.3±2.3）％,P＜0.05;21 d：（96.6±1.6）％ vs（92.1±4.3）％,P＜ 0.05].EGF receptor positive cells area of EGF nanoparticles group was more than that of EGF group [7 d：（49.4±6.5）％ vs（35.1 ±2.8）％,P＜0.05;14 d：（80.2±3.8）％ vs（73.4 ±1.7）％,P＜0.05].Conclusion EGF nanoparticles can upregulate the expression of EGF receptor of cells in wound and accelerate wound healing.The effects are better than that of EGF.

关 键 词：糖尿病 表皮生长因子 纳米微粒 表皮生长因子受体 创面愈合 

分 类 号：R587.2[医药卫生—内分泌]