半夏泻心汤对糖尿病大鼠胃窦Cajal间质细胞及干细胞因子的影响  

Effect of Banxia Xiexin Decoction on Gastric Antral Interstitial Cells of Cajal and Stem Cell Factor in Diabetic Rats

作　　者：蒋楠[1] 余跃[1] 陈凤琴[1] 袁莉莉[1] 王巧民[1] 

机构地区：[1]安徽医科大学附属省立医院消化内科,合肥230001

出　　处：《中国中西医结合杂志》2013年第12期1672-1676,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：安徽省自然科学基金资助项目(No.1208085MH174);安徽省教育厅自然科学研究重点项目(No.KJ2010A192)

摘　　要：目的观察半夏泻心汤对糖尿病(diabetes mellitus,DM)大鼠胃窦Cajal间质细胞(interstitial cells of Cajal,ICCs)及干细胞因子(stem cell factor,SCF)表达的影响。方法将60只健康雄性DM大鼠随机分成对照组、模型组、半夏泻心汤组(中药组)和多潘立酮组(西药组)。一次性腹腔注射链脲佐菌素(STZ,55 mg/kg)制作糖尿病大鼠模型。中药组给予半夏泻心汤[5.4 g/(kg·d)]灌胃,西药组给予多潘立酮[5 mg/(kg·d)]灌胃,模型组及对照组每天给予等体积蒸馏水,持续灌胃8周后,检测各组大鼠体重及血糖,给予半固体营养糊灌胃测定胃残留率,利用免疫组化和图像分析观察胃窦c-kit阳性ICCs及SCF表达。结果与对照组比较,模型组体重减轻,血糖及胃残留率升高,c-kit阳性ICCs及SCF平均光密度值显著降低(P<0.05)。与模型组比较,两个用药组多饮、多食及多尿症状减轻,精神状态好转,体重、c-kit阳性ICCs及SCF平均光密度值明显增加(P<0.05),胃残留率降低(P<0.05);中药组血糖降低(P<0.05),且中药组c-kit阳性ICCs平均光密度高于西药组(P<0.05)。结论半夏泻心汤可以促进糖尿病大鼠胃窦肌间神经丛c-kit ICCs及SCF的表达,对受损的DM大鼠胃窦ICCs、SCF有部分恢复作用,可改善糖尿病大鼠的胃动力障碍。Objective To observe the effect of Banxia Xiexin Decoction （BXD） on expression of the interstitial cells of Cajal （ICCs） and stem cell factor （SCF） in the antrum of rats with diabetes mellitus （DM）. Methods Totally sixty healthy male SD rats were randomly divided into the control group, the model group, the BXD group, and the Western medicine group （WM, treated by domperidone）, 15 in each group. Diabetic rat models were established by a single intraperitoneal injection of streptozotocin （ STZ, 55 mg/kg）. Those in the BXD group were perfused with BXD at the daily dose of 5.4 g/kg. An equal volume of distilled water was given by gastrogavage to those in the WM group and the control group for 8 successive weeks. The body weight and blood glucose of all rats were detected ,and the gastric residual rates were detected with semisolid nutrient paste by gastrogavage. The expression of positive ICCs and SCF were observed by immunohistochemical method and quantified image analyzer. Results Compared with the control group,the body weight reduced ,blood glucose and gastric residual rates increased, and the mean optical density of positive ICCs and SCF significantly decreased in the model group （P 〈0.05）. Compared with the model group,symptoms such as polydipsia,polyphagia, polyuria were relieved, spir- its improved,the body weight and mean optical densities of positive ICCs and SCF significantly increased （P 〈0.05） ,and gastric residual rates significantly decreased in the BXD group and the WM group （P 〈 0.05）. The blood glucose significantly decreased （P 〈0.05） in the BXD group. The mean optical density of positive ICCs was higher in the BXD group than in the WM group （P 〈0.05）. Conclusions BXD could promote the expression of positive ICCs and SCF. It could improve the gastric motility in DM rats by par- tially inversing abnormal changes of gastric antral ICCs and SCF.

关 键 词：糖尿病 半夏泻心汤 CAJAL间质细胞 干细胞因子 

分 类 号：R285.5[医药卫生—中药学]