异种动物代乳用于剖宫产长爪沙鼠种群净化  

Cleansing of Gerbils Through Cesarotomy and Foster Nursing with Rat and Mice

作　　者：韦永芳[1] 梁成结[1] 刘寒英[1] 冯媛瑜[1] 

机构地区：[1]广州医科大学实验动物中心,广州510182

出　　处：《实验动物与比较医学》2013年第6期444-447,共4页Laboratory Animal and Comparative Medicine

基　　金：广东省科技计划项目（20108060500017）

摘　　要：目的探讨长爪沙鼠剖宫产净化种群的代乳方法。方法在超净工作台对妊娠长爪沙鼠进行剖宫取胎，选择有1～2胎哺乳经验的SPF级NIH、SD母鼠代乳，统计沙鼠的胎间隔、胎鼠成活率和离乳率，绘制代乳胎鼠1~4周的生长曲线，并取净化后2月龄的沙鼠按小鼠微生物学和寄生虫学质量控制国家标准进行检测。结果长爪沙鼠的平均胎间隔为30．14士4．40（22-38）d，代乳成活率和离乳率均超过50％，代乳鼠与非代乳鼠的生长曲线在后期无明显差异，微生物学和寄生虫学检测均符合清洁级小鼠的国家标准。结论NIH、SD母鼠代乳剖宫取胎的长爪沙鼠可以净化种群。Objective To Explore methods of foster nursing in the cleansing of gerbils through cesarotomy. Methods Pregnant gerbils for cesarotomy in super-clean bench and the newborn litters were fostered by mother NIH mice and SD rat which have 1-2 litters, skillful suck in barrier system. The litters interval ,the survival rate and the weaning rate of litters which reared by foster nursing were detected, the growth curve of litters for 1-4 weeks were drawn. The baby gerbils were detected at 60-day-old following the state standard for clean mouse. Results The litter interval of gerbils were 30.14 ＋ 4.40 （22-38） d, the survival rate and weaning rate of litter gerbils by foster nursing were over 50%. There were no significant difference in the growth curve between normal suckled gerbils and gerbils by foster nursing for 1-4 weeks. These gerbils were in accordance with the state standard for clean mouse. Conclusion The cleansing of gerbils can be obtained through cesarotomy and foster nursing by using mother ICR mouse and SD rat.

关 键 词：长爪沙鼠 剖宫产 NIH小鼠 SD大鼠 代乳 

分 类 号：R719.8[医药卫生—妇产科学] R-332[医药卫生—临床医学]