裸鼹鼠生化位点的初步研究  

Preliminary Studies on Biochemical Loci of Naked Mole Rat

作　　者：林丽芳[1] 王洪[2] 赵善民[1] 肖邦[1] 孙智远[1] 张璐[1] 袁子彦[1] 于琛琳 蔡丽萍[1] 徐晨[1] 汤球[1] 孙伟[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433 [2]中国食品药品检定研究院,北京100050

出　　处：《实验动物与比较医学》2013年第6期477-480,486,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科技发展基金项目（No.12140900200）}=j（No.13140900400）联合资助

摘　　要：目的研究裸鼹鼠（NMR）的生化位点特征，初步建立裸鼹鼠生化位点的检测方法。方法以小鼠近交系的生化位点为参照，应用小鼠的检测方法，检测裸鼹鼠的11种同工酶的活性。结果裸鼹鼠的酯酶-1、转铁蛋白为慢带；葡萄糖磷酸异构酶-1、苹果酸酶-1、异柠檬酸脱氢酶-1为中间带；血红蛋白B链、肽酶-3、碱性磷酸酶-1、磷酸葡萄糖变位酶-1、酯酶-3、过氧化氢酶．2为快带。结论7只裸鼹鼠的11个生化位点为一致的条带，无多态性，表现为纯合型。Objective A elementary detection method on biochemical marker loci was established to study the characteristics of naked mole rat biochemical loci. Methods With inbred mouse as a reference, 11 isozymes of naked mole rat were detected according to the methods for biochemical markers of mice. Results The esterase-1, transferrin of naked mole rat showed slower bands; glucose phosphate isomerase-1, malic enzyme-l, isocitrate dehydrogenase-1 showed intermediate bands; hemoglobin [3 chain, peptidase-3, alkaline phosphatase-1, phosphate glucose mutase-1, esterase-3, hydrogen peroxide enzyme-2 showed faster bands. Conclusion Biochemical locis of 7 naked mole rat performed consistent bands, showed homozygous, no polymorphism.

关 键 词：裸鼹鼠 生化位点 遗传检测 

分 类 号：Q95-33[生物学—动物学]