苯并(a)芘染毒致人支气管上皮细胞和上皮样成纤维细胞周期的变化  

Change of cell cycle in human bronchial epithelial cells and epithelioid lung fibroblasts exposed to benzo(a) pyrene

作　　者：陈文涛[1] 范燕峰 张慧涛[1] 杨瑾[1] 

机构地区：[1]山西医科大学公共卫生学院劳动卫生学教研室,山西太原030001

出　　处：《癌变．畸变．突变》2013年第6期454-456,460,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81273041;30901180);山西省高等学校优秀青年学术带头人支持计划资助(晋教科【2010】4号)

摘　　要：目的:观察苯并(a)芘[benzo(a)pyrene,BaP]染毒对人支气管上皮细胞(16HBE)和上皮样肺成纤维细胞(W138)细胞周期的影响,并探讨其剂量效应和时间效应。方法:以16HBE和W138细胞未处理组为阴性对照组,二甲基亚砜(DMSO)组为溶剂对照组。分别以不同浓度(1、2、4、8、16、32μmol/L)的BaP染毒16HBE和W138细胞,24 h后用流式细胞术检测细胞周期分布情况;分别以16μmol/L BaP染毒16HBE和W138细胞,作用不同时间后(1、2、4、8、12、24 h)检测细胞周期分布情况;分别以16μmol/L BaP染毒16HBE和W138细胞,作用4 h后,再经过不同时段(0、1、2、4、8、12、24 h)的恢复期后检测细胞周期分布情况。结果:与阴性对照组比较,随着染毒浓度和染毒时间的增加16HBE和W138细胞S期所占比例均增加(P<0.05);16μmol/L BaP染毒16HBE细胞4 h后,经2～12 h的恢复期(正常条件下培养),S期细胞所占比例与刚染毒后细胞(32.43%)相比明显增加(P<0.05),恢复24 h时S期细胞所占比例(24.52%)与阴性对照组(26.41%)相比差异无统计学意义(P>0.05);16μmol/L BaP染毒W138细胞4 h后恢复0～4 h时S期细胞所占比例与阴性对照组(32.42%)相比明显增加(P<0.05),恢复8 h开始减少,24 h时S期细胞所占比例(32.89%)与阴性对照组(32.42%)相比差异无统计学意义(P>0.05)。结论:BaP染毒16HBE和W138细胞引起细胞周期分布变化,主要为S期阻滞,G1期和G2期变化不明显。OBJECTIVE：To explore the dose-and time-dependent effect on cell cycle of human bronchial epithelial cells （16HBE） and epithelioid lung fibroblasts cells （W138） exposed to benzo[a]pyrene. METHODS：The untreated group of 16HBE and W138 cells were used as negative control group and the dimethyl sulfoxide （DMSO） group as solvent control. 16HBE and W138 cells were exposed to different concentrations of BaP （1,2,4,8,16 and 32μmol/L） for 24 hours. 16HBE and W138 cells exposed to 16 μmol/L BaP were collected at different time points （0,1,2,4,8,12 and 24 h）. 16HBE and W138 cells exposed to 16 μmol/L for 4 h were collected after different recovery times （0,1,2,4,8,12 and 24 h）. Flow cytometry was used to assess the cell cycle. RESULTS：Compared with the negative control group,the proportion of S phase cells was significantly increased as BaP exposure dose and time increased （P〈0.05）. With the extension of recovery time 2-12 h （with normol cell culture condition）,the proportion of S phase 16HBE cells increased （P〈0.05） compared with negative group （32.43%）. At 24 h of recovery time the proportion of S phase （24.52%） showed no significance （P〉0.05） compared with negative group （26.41%）. The proportion of S phase W138 cells with the extension of recovery time 0-4 h increased （P〈0.05）,compared with negative group （32.42%）. The proportion of S phase cells decreasd at 8 h of recovery time with no significance between the negative group （32.42%） and recovery 24h group （32.89%）. CONCLUSION：BaP exposure could lead to 16HBE and W138 cell cycle arrested in S phase,but G1 and G2 did not change significantly.

关 键 词：苯并(A)芘 人支气管上皮细胞 上皮样肺成纤维细胞 细胞周期 

分 类 号：Q355[生物学—遗传学]