乌司他丁对肠缺血-再灌注大鼠血清TNF-α,IL-6的影响  

Effects of Ulinastation on Serum TNF-α, IL-6 in Rats after Intestinal Ischemia-reperfusion

作　　者：范玲玲[1] 侯明星[2] 都义日[1] 孙俊枝[1] 丁翠霞[1] 陈冬梅[1] 

机构地区：[1]内蒙古医科大学第一附属医院麻醉科,内蒙古呼和浩特010050 [2]内蒙古医科大学第一附属医院普外科,内蒙古呼和浩特010050

出　　处：《现代生物医学进展》2013年第31期6011-6013,共3页Progress in Modern Biomedicine

摘　　要：目的:通过检测血清TNF-α,IL-6的变化,评价乌司他丁对小肠缺血再灌注损伤的保护作用及机制。方法:健康Wistar大鼠84只,通过夹闭肠系膜上动脉(SMA)60min制作肠缺血模型,随机分成假手术组(C),肠缺血再灌注组(I),UTI治疗组(U)。根据缺血后再灌注时间不同又将I组和U组分成0min、2h和6h组。I组、U组于手术前经尾静脉分别注入生理盐水2mL、乌司他丁5×104U/kg,假手术组仅分离SMA,不夹闭血管。于各时点取腹主动脉血测定血清TNF-α、IL-6含量。结果:肠缺血再灌注各时相点均引起血清TNF-α、IL-6的变化,与假手术组相比,各时点TNF-α值显著升高(P<0.01),IL-6显著升高(P<0.01)。U组0min、2h血清TNF-α值低于相应时点的I组(P<0.01);U组0min、2h、6h血清IL-6值低于相应时点的I组(P<0.05)。结论:乌司他丁可减轻小肠缺血再灌注后的炎症反应。Objective： To evaluate the protective effect and mechanism of ulinastatin on intestinal ischemia-reperfusion injury by detecting the changes of TNF-α IL-6 in serum levels. Methods： Eighty-four healthy Wistar rats were randomly divided into sham operation group （C）, intestinal ischemia-reperfusion group （I）, and the UTI treatment groups 03）. Intestinal ischemia model was produced by occlusion of the superior mesenteric artery （SMA） for 60 min. Then Group I and Group U divided into 0min, 2 h and 6 h group respectively according to the ischemia reperfusion time. In group I and group U, the rats were treated with normal saline （2 mL） UTI （5 - 104U/kg） via the tail vein. In sham-operated group, SMA was separated, but without occlusion. The serum concentration of TNF-α IL-6 in the abdominal aorta was detected at above time point respectively. Results： Intestinal ischemia-reperfusion at each time point resulted in serum TNF-α, 1L-6 changes. Compared with that in the sham group, the serum concentration of TNF-α and 1L-6 in group I and group U increased obviously at all time points （P 〈 0.01）. Compared with the corresponding time point of group I, the serum concentration of TNF-α at 0 min and 2 h in group U decreased significantly （P〈0.01）, and the serum concentration oflL-6 at 0 min, 2 h and 6h in group U decreased （P〈0.05）. Conclusion： Ulinastatin can reduce the inflammatory response in the small intestine ischemia reperfusion.

关 键 词：乌司他丁 小肠 缺血-再灌注 TNF-Α IL-6 

分 类 号：Q95-3[生物学—动物学] R656.7[医药卫生—外科学]