胰岛素对糖尿病兔牙槽骨缺损修复的影响  

Effect of the Treatment with Insulin on the Reparation of Alveolar Bone Defect of Diabetic Rabbits

作　　者：苏鑫[1] 刘继乐[1] 李新[1] 毕良佳[1] 侯传记[1] 胡馨元[1] 

机构地区：[1]哈尔滨医科大学附属第四医院口腔科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2013年第32期6240-6243,6284,共5页Progress in Modern Biomedicine

基　　金：黑龙江省青年科学基金项目(QC2010073)

摘　　要：目的:探讨胰岛素对糖尿病兔牙槽骨缺损修复治疗的效果,为糖尿病所致的牙周炎提供临床治疗的依据。方法:40只大耳白兔随机分为4组:A组制备健康兔的牙槽骨缺损;B组为胰岛素组,制备健康兔牙槽骨缺损后,用胰岛素治疗;C组为糖尿病组,制备糖尿病兔牙槽骨缺损;D组为糖尿病胰岛素治疗组,制备糖尿病兔牙槽骨缺损后,用胰岛素治疗。每组10只,缺损制备后4、8周各处死5只,对各组成骨情况进行组织学观察及测定。结果:组织学观察A、B、D组修复区可见大量新骨形成,以B组为显著;C组仅见少许成骨,多为纤维组织。新生骨面积比和成骨细胞数在4、8周时均为D组大于C组,B组大于A组,组间差异有统计学意义(P<0.05)。证明应用胰岛素促进糖尿病兔缺损牙槽骨形成新骨的效果明显。结论:胰岛素能够促进糖尿病兔牙槽骨缺损的成骨,为临床上治疗糖尿病并发牙周炎提供一种新的手段。Objective： To investigate the effect of insulin on the alveolar bone defects on the treatment of diabetic rabbits so as to provide the basis for clinical treatment. Methods： 40 healthy rabbits were randomly divided into four groups with 10 in each group： Group A： alveolar bone defects were created in the healthy rabbits; Group B： alveolar bone defects were created in the healthy rabbits that treated with insulin; Group C, alveolar bone defects were created after establishing DM model; Group D, alveolar bone defects were created after establishing DM model and treated with insulin. At 4 and 8 weeks after surgery, rabbits were sacrificed respectively. In ad- dition, histological quality and quantity were analyzed to observe histocompatibility and newborn osteogensis. Results： Histologic exami- nation revealed that the defects in group A and D were filled with new bone, Group B presented more new bone formation, while Group C were filled with connective tissues while new bone formated rarely; at 4 and 8 week, the osteoblast number and the rate of defect new bone filled of group B were both higher than those of group A; Similarly, group D were higher than those of group C. It is obvious that in- sulin could promote the new diabetic rabbit alveolar bone defect. Conclusion： Insulin could promote osteogenesis at alveolar bone defects of diabetes rabbits and provide a new means ofperiodontitis for the clinical treatment of diabetes mellitus.

关 键 词：胰岛素 糖尿病 牙槽骨缺损 兔 

分 类 号：Q953[生物学—动物学] R587.2[医药卫生—内分泌]