基于磷含量调控的红霉素发酵培养基优化  

Optimization of Fermentation Medium for Erythromycin by Regulation of Phosphate Content

作　　者：于晓光[1] 陈勇[1,2] 庄英萍[1] 储炬[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《中国医药工业杂志》2013年第12期1217-1221,共5页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2011ZX09203-001-03)

摘　　要：本研究依据有机氮源磷含量不同设计了3种培养基,试验结果表明,低磷培养基(黄豆饼粉2.0%、脱脂豆粉1.0%和羽毛蛋白胨1.0%)条件下红霉素效价较高,为11 209?g/ml。为了降低发酵后期黄豆饼粉中磷的释放所造成的红霉素合成受阻遏,在上述培养基的基础上用0.5%硫酸铵和0.031%磷酸二氢钾替换1.0%黄豆饼粉(替换前后氮磷总量基本相等),红霉素效价达11 657?g/ml,同时发酵液后期黏度约降低20%。Three fermentation media with different phosphate contents were designed. The highest erythromycin titer of 11 209 μg/ml was obtained under the low level phosphate medium containing 2.0% soybean meal, 1.0% defatted soybean powder and 1.0% feather peptone. In order to avoid the repression of erythromycin biosynthesis by phosphate released from the soybean meal, 1.0% soybean meal was substituted by 0.5% ammonium sulfate and 0.031% potassium dihydrogen phosphate. The erythromycin production reached to 11 657 μg/ml. The broth viscosity decreased by 20%.

关 键 词：培养基优化 红霉素 磷含量 无机氮磷 

分 类 号：TQ465[化学工程—制药化工]