三黄片中10种化学成分的HPLC-DAD法测定  

Determination of Ten Chemical Components in Sanhuang Tablets by HPLC-DAD

作　　者：杜倩[1,2] 汤道权[1,2] 倪璐卿[1] 吴晓雯[1] 

机构地区：[1]徐州医学院药学院,江苏徐州221004 [2]徐州医学院新药与临床应用实验室,江苏徐州221004

出　　处：《中国医药工业杂志》2013年第12期1278-1280,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法同时测定三黄片中的10 种化学成分--野黄芩苷、黄芩苷、盐酸小檗碱、黄芩素、芦荟大黄素、汉黄芩素、大黄酸、大黄素、大黄酚和大黄素甲醚。采用Zorbax SB-C18 色谱柱，流动相为乙腈-0.1％磷酸（ 以三乙胺调至pH 3.1），梯度洗脱，二极管阵列检测器检测。结果显示上述10 种化学成分均达到基线分离，线性范围较宽且线性关系良好。回收率为96.8％～ 101.1％，RSD 为0.42％～ 1.84％。An HPLC-DAD method was established for the determination of ten chemical components in Sanhuang tablets, which were scutellarin, baicalin, berberine hydrochloride, baicalein, aloe-emodin, wogonin, rhein, emodin, chrysophanol and physcione. An Zorbax SB-C18 column was used with the mobile phase of acetonitrile-0.1% phosphonic acid （adjusted to pH 3.1 by triethylamine） by gradient elution with DAD detection. The results showed that the ten components were well separated and the linear relationships were good within wide ranges. Their recoveries were 96.8% - 101.1%, with RSDs of 0.42% - 1.84%.

关 键 词：三黄片 高效液相色谱 测定 

分 类 号：O657.72[理学—分析化学] TQ460.72[理学—化学]