不同厂家舒血宁注射液中芦丁和水仙苷的HPLC含量测定  

Quantitative determination of rutin and narcissoside by HPLC in Shuxuening Injections from different manufacturers

作　　者：马志强[1] 刘彬丽[1] 刘璐[1] 蔡程科[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学研究生院

出　　处：《北京中医药大学学报》2013年第11期768-770,共3页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的建立舒血宁注射液中芦丁和水仙苷的HPLC含量测定方法。方法采用DIKMA Diamonsil-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.4%磷酸溶液（35∶65）,检测波长360 nm,流速1.0 mL/min,柱温35℃。结果芦丁在0.101~0.756μg、水仙苷在0.100~0.752μg范围内呈良好线性关系,r均为0.999 5;加样回收率分别为101.1%和102.0%。结论方法简便准确,灵敏度高,重复性好,可用于舒血宁注射液中芦丁和水仙苷的质量控制。Objective To establish a HPLC method for quantitative determination of rutin and narcissoside in Shuxuening Injection. Methods The HPLC procedure was performed on the chromatographic column of DIKMA Diamonsil-C18 （250 mm×4.6 mm, 5μm）at 35 ~C , the mobile phase was methanol-0.4% phosphoric acid solution （35：65 ） with the flow velocity of 0.1 mL/min, and the detection wavelength was 360 nm. Results Rutin showed a linear relationship at a range from 0. 101μg to 0. 756 μg （ r = 0. 999 5 ） and the recovery was 101.1%. Narcissoside showed a linear relationship at a range from 0. 100 μg to 0. 752 μg （ r = 0. 999 5 ） and the recovery was 102.0%. Conclusion The method is simple, accurate with high sensitivity and good repeatability, and can be used for the quality control of rutin and narcissoside in Shuxuening Injections.

关 键 词：舒血宁注射液 芦丁 水仙苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]