某规模化猪场猪伪狂犬病的诊断  

Diagnosis of Swine Pseudorabies in One Large Scaled-swine Farm

作　　者：杨志远[1,2] 韩春华[2] 林健[2] 赵景义[3] 李栋梁[3] 刘月焕[2] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京市农林科学院畜牧兽医研究所,北京100097 [3]北京市畜牧兽医总站,北京100107

出　　处：《中国畜牧兽医》2013年第12期185-189,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31072159)

摘　　要：试验采用流行病学调查、临床观察和实验室检查对华北地区某疑似伪狂犬病发病猪场进行了诊断。猪场发病情况主要为母猪流产,新生仔猪死亡率较高,肥猪死亡率低。发病或死亡仔猪扁桃体充血出血、溃疡,肺脏出血、水肿,心肌色淡、心包积液,脑膜充血;表现为坏死性扁桃体炎、出血性淋巴结炎、出血性间质性肺炎、间质性心肌炎和非化脓性脑炎。发病猪扁桃体、肺脏、脑组织匀浆上清液接种家兔后出现奇痒等典型症状并很快死亡。发病猪脑组织可检出猪伪狂犬病毒gD基因特异性目的片段。猪场伪狂犬病野毒感染抗体阳性率为63%(63/100)。本试验结果表明,确诊该猪场发生了伪狂犬病。To diagnose an infectious disease suspected as swine pseudorabies （PR） in swine farm in North China, epidemiological investigation, clinical observation and laboratory examination were performed. High mortality in piglets, abortion in sows and low mortality in finishing pigs were major clinical signs. Tonsil hyperaemia and ulcer, pneumonedema, increased pericardial effusion and meningeal congestion were observed in piglets. Histopathological microscopies were as necrotizing tonsillitis, hemorrhagic lymphadenitis, interstitial pneumonia, interstitial myocarditis, and nonsuppurative encephalitis. Rabbits were injected with the tonsil, lung and brain tissue homogenate supernatant of pigs with the clinical symptom, and the typical clinical syndrome such as extra tickle and acute death could be observed. The pseudorabies virus （PRV） gD gene specific fragment was amplified from DNA extracted in piglet brain by PCR. The result of serologic survey demonstrated that 63% （63 of 100） pigs were positive for antibodies against PRV. The results showed that PRV infection was the etiologic agents in this swine farm.

关 键 词：猪 伪狂犬病 诊断 

分 类 号：S852.65[农业科学—基础兽医学]