Culture Experiment in vitro of Eperythrozoon of Mustela lutreola  

水貂附红细胞体的体外培养试验(英文)

作　　者：高光平[1] 高桂生[1] 史秋梅[1] 张艳英[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《Agricultural Science & Technology》2013年第11期1636-1638,1641,共4页农业科学与技术（英文版）

基　　金：Supported by Project of Hebei Science and Technology Department(10960408D01)~~

摘　　要：[Objective] The research aimed to make the culture experiment in vitro of eperythrozoon of Mustela lutreola. [Method] 30% newborn bovine serum or Mustela lutreola was added in RPMI-1640, DMEM, M-199 and HL culture solutions. Epery-throzoon of M. lutreola was cultured in vitro under the conditions of 37 ℃, 5% CO2. The culture solutions were replaced every 24 hours. Appropriate amount of healthy erythrocytes were supplemented to make subculture. [Result] The infection rate and infection intensity of eperythrozoon in RPMI-1640 and HL culture solutions were higher than that in DMEM and M-199 culture solutions. The infection rate and infec-tion intensity of eperythrozoon in RPMI-1640 and HL culture solutions with adding 30% serum of M. lutreola were higher than that with adding 30% newborn bovine serum. And the infection rate and infection intensity of eperythrozoon in RPMI-1640 culture solution were slightly higher than that in HL culture solution. The infection rate was over 80% after culturing 12 hours. 23 generations of subculture in vitro was made. [Conclusion] The research provided an effective approach for screening out the drugs that could effectively control eperythrozoon of M. lutreola, preparing diagnostic antigen and producing the relevant vaccine.[目的]对水貂附红细胞体进行体外培养试验。[方法]在RPMI-1640、DMEM、M-199和HL培养液中添加30%的新生牛血清或水貂血清,在37℃、5%CO2条件下体外培养水貂附红细胞体,每12 h更换1次培养液,补充适量健康水貂红细胞,并进行传代培养。[结果]RPMI-1640和HL培养液中附红细胞体的感染率和感染强度高于DMEM和M-199培养液,在RPMI-1640和HL培养液中添加30%水貂血清附红细胞体的感染率和感染强度高于添加30%新生牛血清,且RPMI-1640略高于HL培养液,培养12 h后获得了80%以上的感染率,且体外传代可连续培养23代。[结论]该研究为筛选有效防治水貂附红细胞体的药物及制备诊断抗原与研制疫苗提供了有效的途径。

关 键 词：M-ustela lutreola EPERYTHROZOON Culture in vitro 

分 类 号：S858.92[农业科学—临床兽医学]