多聚赖氨酸修饰SPIO标记骨髓间充质干细胞体外磁共振成像研究  

Research of MRI in vitro of polylysine modified SPIO labeled BMSCs

作　　者：刘慧慧[1] 谢晓东[1] 吴荧荧 刘庆[1] 陈芳芳[1] 蔡小威 许云飞[1] 张礼荣[1] 朱海涛[1] 

机构地区：[1]江苏大学附属医院放射科,江苏镇江212013

出　　处：《现代医药卫生》2013年第24期3681-3683,共3页Journal of Modern Medicine & Health

基　　金：江苏省自然科学基金(BK2011487);江苏大学医学临床科技发展基金(JLY2010154);江苏大学大学生科研立项资助项目(12A211)

摘　　要：目的利用多聚赖氨酸(PLL)修饰的超顺磁性氧化铁粒子(SPIO),即超顺磁性氧化铁纳米粒子(SPIOPLL)标记大鼠骨髓间充质干细胞(BMSCs),探讨其对BMSCs生物学特性的影响以及3.0 T磁共振成像(MRI)上T2信号的改变。方法将传代培养BMSCs与不同铁浓度的SPIO-PLL溶液共同孵育24 h后行普鲁士蓝染色,观察BMSCs对不同浓度SPIO-PLL的摄取,确定SPIO-PLL标记干细胞的最佳浓度。同时采用四唑盐比色法分析不同浓度SPIO-PLL溶液对大鼠BMSCs生长活性的影响。取最适浓度的SPIO-PLL标记BMSCs,经3.0 T MRI,观察T2WI信号的改变。结果 SPIOPLL标记BMSCs生物活性没有明显影响,与未标记细胞的生长活性比较,差异无统计学意义(P>0.05);同时铁浓度不同(10、40、80μgFe/mL)的SPIO-PLL溶液标记细胞成像可使T2WI图像信号降低,3种浓度的信号变化率比较,差异有统计学意义(P<0.05)。结论 SPIO-PLL可以标记BMSCs,且对BMSCs的生物学活性没有影响,MRI T2WI序列可敏感显像磁性标记的干细胞,有望为MRI和光学成像活体示踪BMSCs提供技术基础。Objective To label rats bone marrow stem cells（BMSCs） with polylysine（PLL） modified superparamagnetic iron oxide （SPIO） nanoparticles, that is superparamagnetic iron oxide nanoparticals and poly-L-lysine （SPIO-PLL）, and to explore the signal changes ofT2 by 3.0 T magnetic resonave imaging（MRT）. Methods Subcuhuring BMSCs was incubated with different concertrations of SPIO-PLL solution. Prussian blue stain was explored 24 hours after co-culturing to observe the intake of BMSCs to different concentration of SPIO-PLL, and determine the optimal concentration for labeling stem cells by SPIO-PLL. MTY assay was used to analyze the influence of different concentration of SPIO-PLL solution on growth activity of BMSCs. Optimal concentra- tion of SPIO-PLL was adopted to label BMSCs, and the change of T2WI signal was observed through 3.0 T MRI. Results There was no obvious impact on biological activity of BMSCs labeled by SPIO-PLL and compared with growth activity of non-labeled cells, the difference had no statistical significance （P〉0.05） ;labeled cell imaging in different concentration of iron （ 10,40,80 μgFe/mL） in SPIO-PLL solution could reduce the T2WI image signal, and the rate of signal change among the concentrations of solution had statistically significant difference （P〈0.05）. Conclusion SPIO-PLL can label BMSCs, and it has no effect to the biological activity of BMSCs. MRI T2WI sequence can sensitively develop the SPIO labeled stem cells, and it can provide technological basis for MRI and optical imaging in vivo tracking of BMSCs.

关 键 词：聚赖氨酸 纳米粒子 生物学标记 间质干细胞 磁共振成像 仪器和设备 磁力学 铁 信号传导 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]