银中杨落叶的化学成分及活性研究  

Chemical constituents from fallen leaves of Populus alba × P.berolinensis

作　　者：王金兰[1] 孙大鹏[1] 吕伟强[1] 李军[1] 赵明[1] 张树军[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《中草药》2013年第23期3276-3281,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的研究银中杨Populus alba×P.berolinensis落叶的化学成分及水杨苷衍生物对SGC-7901细胞的抑制活性。方法采用硅胶柱色谱和高效液相色谱等进行分离纯化,依据理化性质及波谱数据分析进行结构鉴定,利用MTT法对水杨苷衍生物进行人胃癌SGC-7901细胞体外增殖的抑制活性实验。结果从银中杨落叶中分离得到16个化合物,分别鉴定为颤杨苷(1)、白杨苷(2)、3′-O-苯甲酰基水杨苷(3)、4′-O-苯甲酰基水杨苷(4)、水杨苷(5)、特里杨苷(6)、poliothrysin benzoate(7)、邻苯二酚(8)、苯甲酸(9)、tremulacinol(10)、6′-O-苯甲酰基水杨苷-7-水杨酸酯(11)、水杨醇(12)、salicortin(13)、7-O-乙酰基-3′-O-苯甲酰基水杨苷(14)、7-O-乙酰基-4′-O-苯甲酰基水杨苷(15)、6′-O-乙酰基-2′-O-苯甲酰基水杨苷(16)。MTT实验表明,特里杨苷类衍生物化合物7、11～13对SGC-7901细胞体外增殖均显示一定抑制活性,IC50分别为71.0、122.5、212.7、257.8μmol/L。结论化合物4、7、10、11、14～16为首次从该植物中分离得到,化合物7对人胃癌SGC-7901细胞体外增殖具有抑制作用。Objective To study the chemical constituents from the fallen leaves of Populus alba ×P. berolinensis and the inhibitory activity of salicin derivatives against SGC-7901 cell. Methods The chemical constituents were isolated and purified on the basis of silica gel column chromatography and HPLC. The structural elucidation was performed according to the physicochemical properties and spectroscopic analysis. The inhibitory activity of salicin derivatives on human gastric carcinoma cells SGC-7901 in vitro proliferation was determined by MTT method. Results Sixteen compounds were isolated and identified as tremuloidin （1）, populin （2）, chaenomeloidin （3）, 4＇-O-benzoylsalicin （4）, salicin （5）, tremulacin （6）, poliothrysin benzoate （7）, catechol （8）, benzoic acid （9）, tremulacinol （10）, 6＇-O-benzoylsalicin-7-salicylate （11）, salicylol （12）, salicortin （13）, 7-O-acetyl-3＇-O-benzoylsalicin （14）, 7-O-acetyl-4＇-O-benzoylsalicin （15）, and 6＇-O-acetyl-2＇-O-benzoylsalicin （16）, respectively. The inhibitory activity of compounds 7 and 11--13 on the in vitro proliferation of SGC-7901 cell was indicated by MTT and IC50 values were 71.0, 122.5, 212.7, and 257.8 μmol/L. Conclusion Compounds 4, 7, 10, 11, 14--16 are isolated from this plant for the first time. Compound 7 shows the inhibitory activity against SGC-7901 in vitro proliferation.

关 键 词：银中杨 白杨苷 水杨苷 特里杨苷 水杨醇 7-O-乙酰基 3’-O-苯甲酰基水杨苷 

分 类 号：R284.1[医药卫生—中药学]