蒙古黄芪主产区2种不同种植模式黄芪药材的质量比较  

Comparative study on quality of Astragali Radix by two different planting patterns in major producing areas

作　　者：杜国军[1,2] 秦雪梅[2] 李震宇[1] 何盼[1,2] 高凡茸 李科[1] 周然[3] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]山西中医学院,山西太原030024

出　　处：《中草药》2013年第23期3386-3393,共8页Chinese Traditional and Herbal Drugs

基　　金：国家"十二五"科技支撑计划(2011BA107B01);山西省科技攻关项目(20100312027-6);山西省中药材;中药饮片地方标准研究(2011014A)

摘　　要：目的比较传统与2年生栽培蒙古黄芪药材的化学成分,探讨化学成分差异性。方法采集HPLC-ELSD指纹图谱信息,测定指标成分黄芪甲苷、毛蕊异黄酮葡萄糖苷、总多糖、浸出物的量。对传统黄芪和2年生栽培黄芪样品进行指纹图谱相似度分析、代谢组学多元统计分析以及质量比较。结果传统黄芪的毛蕊异黄酮葡萄糖苷和总多糖量明显高于2年生栽培黄芪,但二者的黄芪甲苷量和浸出物量没有差别;HPLC-ELSD代谢组学分析技术可以将传统黄芪与2年生栽培黄芪明显区分开,引起二者明显区别的主要差异性化学成分为毛蕊异黄酮葡萄糖苷、芒柄花苷、峰4和5、黄芪皂苷Ⅱ、峰13、黄芪皂苷Ⅰ。结论基于HPLC-ELSD代谢组学技术结合化学指标成分的测定,可用于传统黄芪与2年生栽培黄芪的质量评价研究。Objective To compare the chemical constituents in traditional planted and two-year-old cultivated Astragali Radix, and to discuss the chemical differences. Methods The HPLC-ELSD fingerprint of Astragali Radix was collected, and the contents of astragaloside, calycosin-7-O-13-D-glucoside, total polysccharide, and extract were also determined. The similarity analysis, metabolomic multivariate statistical analysis, and quality comparison were performed. Results The contents of calycosin-7-O-[^-D- glucoside and total polysaccharide in traditional planted Astragali Radix were obviously higher than those in two-year-old cultivated Astragali Radix. But the contents of astragaloside and extract showed no difference. The two kinds of Astragali Radix could be distinguished by HPLC-ELSD metabolomic analysis. The differential metabolites were calycosin-7-O-13-D-glucoside, ononin, peaks 4, 5, 13, astragalosides I and II. Conclusion HPLC-ELSD metabolomic approach combined with the content determination of index components could be used for the quality evaluation of traditional planted and two-year-old cultivated Astragali Radix.

关 键 词：蒙古黄芪 指纹图谱 代谢组学 多元统计分析 HPLC—ELSD 

分 类 号：R282.21[医药卫生—中药学]