Inhibitive effect on phagocytosis of Candida albicans induced by pretreatment with quercetin via actin cytoskeleton interference  

作　　者：Shuna Cui Jing Qian Ping Bo 

机构地区：[1]Department of Integrated Traditional Chinese Medicne and Western Medicine, Medical College of Yangzhou University [2]Biologic Systems Analysis Group, Helmholtz Centre for Infection Research [3]Department of Integrated TCM and Western Medicine, Medical College of Yangzhou University

出　　处：《Journal of Traditional Chinese Medicine》2013年第6期804-809,共6页中医杂志（英文版）

基　　金：Supported by Grants from Scientific Research Foundation of the Higher Education Institutions of Jiangsu Province,China(No.13KJB310022);Administration of Traditional Chinese Medicine of Jiangsu Province(No.LZ13248);China Postdoctoral Science Foundation(No.2013M541741)

摘　　要：OBJECTIVE： To investigate the anti-inflammatory effect of pretreatment with quercetin on macro- phages after Candida albicans infection. METHODS： RAW 264.7 macrophages were used as a target cell line. Cell viability after treatment with quercetin at different time points was detected by Carboxyfluorescein diacetate, succinimidyl ester. Phagocytic function of macrophages was deter- mined by a fluorometric assay. Cytokine tumor ne- crosis factor a （TNF-a） production was measured by enzyme-linked immunosorbent assay. F-actin cy- toskeleton of L929 cells was stained by Alexa Fluor 488-phalloidin. RESULTS： Pretreatment with quercetin decreasedcell viability only at the highest concentration of 37 μg/mL 2, 24, and 48 h after the treatment. The phagocytic efficiency of macrophages pretreated with quercetin was significantly decreased in a time- and dose-dependent manner. F-actin label- ing showed that the actin cytoskeleton of the cells started to break down 2 h after treatment. More- over, it notably inhibited cytokine TNF-a produc- tion after Candida albicans infection. CONCLUSION： Pretreatment with quercetin in- duced an anti-inflammatory effect against Candida albicans infection in macrophages.OBJECTIVE: To investigate the anti-inflammatory effect of pretreatment with quercetin on macrophages after Candida albicans infection. METHODS: RAW 264.7 macrophages were used as a target cell line. Cell viability after treatment with quercetin at different time points was detected by Carboxyfluorescein diacetate, succinimidyl ester. Phagocytic function of macrophages was determined by a fluorometric assay. Cytokine tumor necrosis factor α(TNF-α) production was measured by enzyme-linked immunosorbent assay. F-actin cytoskeleton of L929 cells was stained by Alexa Fluor 488-phalloidin. RESULTS: Pretreatment with quercetin decreased cell viability only at the highest concentration of 37 μg/mL 2, 24, and 48 h after the treatment. The phagocytic efficiency of macrophages pretreated with quercetin was significantly decreased in a timeand dose-dependent manner. F-actin labeling showed that the actin cytoskeleton of the cells started to break down 2 h after treatment. Moreover, it notably inhibited cytokine TNF-α production after Candida albicans infection. CONCLUSION: Pretreatment with quercetin induced an anti-inflammatory effect against Candida albicans infection in macrophages.

关 键 词：QUERCETIN Candida albicans Morpholo-gy PHAGOCYTOSIS ACTINS 

分 类 号：R285[医药卫生—中药学]