GC-TQ-MS/MS测定生理盐水注射液中双酚A  

Determination of bisphenol A in physiological saline injection by GC-TQ-MS/MS

作　　者：陈雪峰[1,2,3] 蒋俊[2,3] 丁敏[4] 吴得利[5] 余劼[2] 

机构地区：[1]太仓市中医医院,江苏太仓215400 [2]南京中医药大学药学院,江苏南京210029 [3]镇江出入境检验检疫局,国家食品添加剂及调味品检测重点实验室,江苏镇江212008 [4]中国人民解放军359医院,江苏镇江2120085 [5]江苏省中西医结合医院,江苏南京210028

出　　处：《药物评价研究》2013年第6期460-462,共3页Drug Evaluation Research

基　　金：江苏出入境检验检疫局项目(2011KJ48);镇江市科技支撑计划(SH2012010)

摘　　要：目的建立生理盐水注射液中双酚A（BPA）残留量的测定方法。方法采用气相色谱-三重四级杆（GC-TQ-MS/MS）联用技术,测定了5批生理盐水注射液中BPA的残留量,每批平行测定6次,且每次分别在5、10、20μg/kg 3个添加水平下进行加样回收,每水平分别测定3次。结果所测5批生理盐水注射液中BPA质量分数分别为27.01、11.80、15.73、22.28、9.03μg/kg;RSD分别为2.3%、4.2%、3.2%、4.4%、1.9%;平均加样回收率在79.9%-9.3%,RSD均小于4.1%。BPA在10-200μg/L内呈良好的线性关系,检测限为1.25μg/kg。结论该法简便快速、灵敏性高,可用于生理盐水注射液中BPA残留量的测定。Objective To establish a GC-TQ-MS/MS method for the determination of bisphenol A（BPA） in physiological saline injection（PSI）.Methods The determination of BPA remaining in five batches of PSI with six parallel tests for each batch.Spiked recovery rates were determined in added levels at 5,10,and 20 μg/kg for three times in each level.Results The contents of BPA in five batches of PSI were 27.01,11.80,15.73,22.28,and 9.03 μg/kg,respectively,with RSD values of 2.3%,4.2%,3.2%,4.4%,and1.9%.The average recovery rates were between 79.9% and 99.3% with RSD values less than 4.1%.There was a good liner relationship in the range of 10—200 μg/L for BPA and the limit of detection（LOD） was 1.25 μg/kg.Conclusion The method is simple,rapid,accurate and repeatable,and can be used to measure the content of BPA in PSI with high sensitivity.

关 键 词：GC-TQ-MS MS 双酚A 生理盐水注射液 

分 类 号：R927.11[医药卫生—药学]