缺氧缺血性脑病新生大鼠海马EphA5受体mRNA表达与神经元凋亡的关系  

Relationship between EphA5 Receptor mRNA Expression and Apoptosis of neurons in Hippocampus of Neonatal Rat with Hypoxic-Ischemic Encephalopathy

作　　者：周永新[1] 刘俊[1] 李海英 吴尤佳[2] 

机构地区：[1]常熟市第二人民医院儿科,江苏常熟215500 [2]南通大学附属医院儿科,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2013年第11期1521-1523,1528,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省研究生创新科研计划(CXZZ12-0838);南通市科技计划项目(BK2011051)

摘　　要：目的:探讨缺氧缺血性脑病(HIE)新生大鼠海马EphA5受体mRNA表达与神经元凋亡的关系。方法:7日龄SD大鼠制备HIE经典模型。分为HIE组、HIE+EphA5-Fc组和对照组。采用qRT-PCR法测定各组新生大鼠海马EphA5受体mRNA表达,原位缺口末端标记(TUNEL)法检测其凋亡的神经元。结果:①HIE组海马EphA5受体mRNA表达上调,在12 h达高峰,48 h仍高于对照组(P<0.05);与HIE组比较,HIE+EphA5-Fc组海马EphA5受体mRNA表达下调(P<0.05),已恢复至对照组水平(P>0.05);②HIE组海马神经元凋亡6 h开始升高,24 h达高峰;HIE+EphA5-Fc组海马神经元凋亡较HIE组显著下降(P<0.05)。结论:阻断EphA5受体可抑制HIE新生大鼠海马EphA5受体mRNA表达上调及海马神经元凋亡,表明EphA5受体活化是缺氧缺血致脑损伤的机制之一。Objective：To explore the mRNA expression of EphA5 receptor in hippocampus of neonatal rat with hypoxic-ischemic encephalopathy （HIE） as well as the relationship with apoptosis of neurons. Methods：Seven-day-old SD rats were randomly divided into HIE group,HIE＋ EphA5-Fc group and control group . The mRNA expression of EphA5 receptor and the apoptosis of neurons in hippocampus of neonatal rats in 3 groups were determined by qRT-PCR and TUNEL staining. Results：①The mRNA expression of EphA5 receptor in HIE group increased and peaked at 12h,which was still higher than that in control group at 48 h （P 〈 0.05）. Compared with HIE group,the mRNA expression of EphA5 receptor decreased in HIE＋ EphA5-Fc group （P 〈 0.05）,which recovered to that in control group （P 〉 0.05）. ②In HIE group,apoptosis of hippocampus neurons increased after 6 h,and peaked at 24 h. Apoptosis of hippocampus neurons in HIE＋ EphA5-Fc group was remarkably lower than that in HIE group （P 〈 0.05）. Conclusion：The up-regulation of EphA5 receptor and apoptosis of hippocampus neurons could be suppressed by inhibitor of EphA5 receptor,which suggests the activity of EphA5 receptor may be one of the mechanisms of brain damage by hypoxic-ischemic.

关 键 词：EphA5受体 凋12 缺氧缺血性脑病 大鼠 海马 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]