miR-518d在正常妊娠及妊娠期糖尿病患者胎盘及血清中的表达研究  

Investigation on the expression of miR-518d in the placenta and serum of gestational diabetes mellitus and normal pregnant women

作　　者：薛璇[1] 王琪[1] 石中华[1] 赵纯[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院产科,南京江苏210004

出　　处：《南京医科大学学报（自然科学版）》2013年第11期1572-1575,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81000258;81100436);江苏省自然科学基金(BK2010586)

摘　　要：目的:检测正常妊娠及妊娠期糖尿病患者胎盘及血清中microRNA518d(miR-518d)的表达,探讨其在妊娠期糖尿病发病及诊断中的作用。方法:选择无妊娠并发症和合并症的足月剖宫产分娩产妇20例及妊娠期糖尿病足月剖宫产分娩产妇20例。分别收集两组的新鲜胎盘组织及母体血清,用Taqman实时荧光定量PCR方法检测两组miRNAs的变化。结果:miR-518d在妊娠期糖尿病胎盘中的表达(4.35±0.88)显著高于对照组(1.04±0.73)(P<0.01),而其在妊娠期糖尿病血清中的表达水平(2.43±1.07)也明显高于对照组(0.96±0.88)(P<0.05),生物信息学预测过氧化物酶体增殖物激活受体α(PPARα)3′UTR区含有miR-518d的结合位点,且胎盘miR-518d表达水平和血清雌二醇水平呈正相关(R2=0.759,r=0.871)。结论:miR-518d可能通过调控PPARα基因参与妊娠期糖尿病的发病,有望成为妊娠期糖尿病的潜在诊断指标。Objective：To investigate the expression level of miR-518d in the placenta and maternal serum of gestational diabetes mellitus and normal pregnant women. Methods：The expression of miR-518d in placenta and serum was measured by Taqman RT-PCR in 20 women with GDM and 20 women with normal term pregnancy. Results：Expression of miR-518d in placenta was significantly up-regulated in GDM group（4.35 ± 0.88）compared to control group（1.04 ± 0.73）（P 〈 0.01）. Expression of miR-518d in serum was also up-regulated in GDM group（2.43 ± 1.07）compared to control group（0.96 ± 0.88）（P 〈 0.05）. We also showed that PPARa was a direct target of miR-518d with a specific binding site at the seed sequence. And expression of miR-518d in the placenta was significantly positive correlated with the expression of serum E2（R2=0.759,r = 0.871）. Conclusion：miR-518d is related with the mechanism of GDM and we suggest that upregulation of miR-518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.

关 键 词：miR-518d 妊娠期糖尿病 雌二醇 胎盘 过氧化物酶体增殖物激活受体Α 

分 类 号：R714[医药卫生—妇产科学]