HPLC法测定骨痹舒片中士的宁和马钱子碱的含量  

On the Determination of the Contents of Strychnine and Brucine in Gubishu Tablet by HPLC Method

作　　者：杨艳娇[1] 刘雅敏[1] 白月明[1] 何永超[1] 马庆峰 

机构地区：[1]河南中医学院,河南郑州450008 [2]河南风湿病医院河南风湿病研究所,河南郑州450045

出　　处：《风湿病与关节炎》2013年第12期39-41,43,共4页Rheumatism and Arthritis

基　　金：河南省基础与前沿技术研究项目(112300410098)

摘　　要：目的：建立高效液相色谱（HPLC）法测定骨痹舒片中士的宁和马钱子碱的含量。方法：采用HPLC法测定Agilent C18色谱柱（4.6 mm×250 mm，5μm），流动相：甲醇250 g·L^-1∶缓冲液（水∶乙酸∶三乙胺=230∶2.4∶0.3）750 g·L^-1；体积流量：1.0 mL·min-1；检测波长254 nm；柱温25℃。结果：士的宁在0.1208～1.4496μg线性关系良好，相关系数为0.9999，士的宁平均回收率为99.01%，RSD为0.91%；马钱子碱在0.0948～1.1376μg线性关系良好，相关系数为1，马钱子碱平均回收率为97.37%， RSD为1.26%。结论：该方法快速、准确、重现性好，可用于骨痹舒片中士的宁和马钱子碱的质量控制。Objective：To establish the HPLC method for determination of strychnine and brucine in gubishu tablet.Methods：By the method of HPLC,Chromatographic column Agilent C18（4.6 mm × 250 mm,5μm）;the mobile phase：was 250 g·L^-1 methanol：750 g·L^-1 buffer solution（water：acetic acid：triethylamine=230：2.4：0.3）; volume lfow ：1.0 mL min-1;detected wavelength：254 nm;column temperature：25℃.Results：The linear relationship of strychnine was good in 0.1208 - 1.4496 μg with the correlation coefficient 0.999 9, the average recovery 99.01% and RSD 0.91%.The linear relationship of brucine in 0.0948 - 1.1376 μg was good,with the correlation coefifcient 1, the average recovery 97.37% and RSD 1.26%.Conclusion：This method is rapid,accurate with good reproducibility,which can be used for the quality control of strychnine and brucin in gubishu tablet.

关 键 词：马钱子碱 士的宁 骨痹舒片 HPLC 

分 类 号：R286[医药卫生—中药学]