恶臭假单胞菌对碳酸钙的诱导矿化作用  

Induced Mineralization of calcium carbonate by Pseudomonas putida

作　　者：韩金鑫[1,4] 连宾[2] 唐源[3] 刘世荣[3] 龚国洪[3] 

机构地区：[1]贵州大学喀斯特环境与地质灾害防治教育部重点实验室,贵阳550003 [2]南京师范大学生命科学学院,江苏省微生物工程技术研究中心,江苏省功能微生物与功能基因组学重点实验室,南京210046 [3]中国科学院地球化学研究所环境地球化学国家重点实验室普定喀斯特生态系统观测研究站,普定562100 [4]中国环境科学研究院湖泊生态环境创新基地,北京100012

出　　处：《南京大学学报（自然科学版）》2013年第6期681-688,共8页Journal of Nanjing University（Natural Science）

基　　金：国家重大科学研究计划(2013CB956702);环境地球化学国家重点实验室科研专项(SKLEG2013407)及开放课题(SKLEG2013816)

摘　　要：本文研究一株分离自白云岩表面的恶臭假单胞菌DCB13菌株对CaCO3的诱导矿化作用．将恶臭假单胞菌DCB13菌株接种到修改后的牛肉膏蛋白胨液体培养基（牛肉膏3g、蛋白胨10g、NaCl5g、CaCl2 2g、玻璃粉2g、蒸馏水1L、pH6．5～7．5）中培养7d，每24h取培养基上清液10mL，离心后用于测定培养液pH值、碳酸酐酶（CA）活性、HCO3^-及Ca^2＋浓度，借助X-射线衍射（XRD）、透射电子显微镜（TEM）及能谱分析（EDS）等方法分析培养7d后培养液沉淀物中是否有CaCO3生成；用不同CaCl2浓度的牛肉膏蛋白胨培养基培养该菌，培养7d后用称量法测定CaCO3的生成量，并计算Ca^2＋固定为CaCO3的转化率．实验结果表明，接人恶臭假单胞菌能提高该培养液的pH值，培养液中HCO3^-及Ca^2＋浓度随培养时间有显著下降的趋势，XRD、TEM均检测出培养液底部沉淀物中有生物成因CaCO3生成，Ca^2＋固定为CaCO3的转化率可达25％以上．该研究结果对碳酸盐的微生物诱导合成研究提供了新的线索．Pseudomonas putida strain DCB13 which isolated from dolomite surface were researched on the inducedmineralization of CaCO3. The strain DCB13 was inoculated into the heel extract peptone liquid modified culture medium（beef extract 3 g,peptone 10 g,NaC1 5 g,CaCl2 2 g,glass powder 2 g, distilled water 1 L,pH 6.5~7.5）for cultured 7-days,sample volumes of 10 mL were taken from the culture medium every 24 h for processing by centrif- ugation,for the pH, CA, HCO7 and Ca2＋ measurement. Precipitation in the culture medium was analyzed to confirm, or otherwise, the presence of CaCO3. Methods used included X-ray diffraction （XRD）, Transmission Electron Microscopy（TEM）, and Energy Dispersive Spectroscopy（EDS）. Medium cultured by setting different CaCIz concentration of beef extract peptone culture, after 7-day cultivation period, by weighing method for the determination of the amount of CaCO3, and calculate the conversion rate from Ca2＋ to CaCO3. The experimental results show that, the P. putida can improve the pH value in this culture medium, concentrations of HCO3- and Ca2＋ showed a significant decline over the duration of the cultivation period. XRD and TEM analysis all revealed the presence of CaCO3 as a precipitate. The conversion rate of Ca2＋ to CaCO3 is higher than 25%. The result of the study provides a new clue on carbonate microbial induced synthesis.

关 键 词：恶臭假单胞菌 碳酸钙 生物成矿 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]