沙棘黄酮对粥样硬化大鼠NADPH氧化酶亚基Nox4及ox-LDL的影响  

Effect of Regulation of TFH on Expression of Nox4 and ox-LDL in Atherosclerosis Rats

作　　者：王颖超[1] 柳茵[2] 刘维军[2] 李琳[2] 

机构地区：[1]青海大学医学院,青海西宁810001 [2]青海大学附属医院,青海西宁810001

出　　处：《云南中医学院学报》2013年第5期7-9,共3页Journal of Yunnan University of Traditional Chinese Medicine

摘　　要：目的探讨沙棘黄酮(TFH)对粥样硬化大鼠NADPH氧化酶亚基Nox4及ox-LDL的影响及机制。方法将50只SD大鼠随机分为:正常对照组(CON组)、粥样硬化组(AS组)、沙棘黄酮低、中、高剂量组,除CON组其余4组喂高脂饲料,TFH低、中、高剂量组以不同剂量TFH灌胃,60 d后,取血清测定SOD、MDA及ROS水平,ELISA技术测主动脉ox-LDL和Nox4蛋白表达水平。结果 AS组比CON组SOD下降,MDA及ROS含量升高;沙棘黄酮干预能改善以上指标;沙棘黄酮降低了AS大鼠Nox4及oxLDL蛋白表达水平。结论沙棘黄酮可降低AS大鼠Nox4及ox-LDL蛋白表达从而减少ROS生成防治粥样硬化。Objective To investigate whether TFH improve the atherosclerosis rats vascular function and its mechanism. Methods 50 male SD rats were divided into 5 groups：blank control group,atherosclerosis group,TFH low,medium and high dose group,After feeding for 60 days. Taking aorta to detect vascular injury condition;Separation and determination of SOD,MDA and ROS content. Elisa technique to detect NADPH oxidase subunit protein Nox4 and ox-LDL expression level. Results Compared AS group to CON,MDA and ROS activity were significantly increased,SOD content decreased significantly;TFH intervention can improve the above indicators;TFH reduce NADPH oxidase protein Nox4 and ox-LDL protein expression level. Conclusion TFH has protective effect of vascular in atherosclerosis rats,Its mechanism could be by lowering Nox4 and ox-LDL protein levels,and reduce ROS generation.

关 键 词：沙棘黄酮 动脉粥样硬化 NOX4 ox—LDL ROS 

分 类 号：R285[医药卫生—中药学]