HPLC-MS/MS法研究大鼠胃内容物对酸枣仁皂苷A的体外代谢作用  

Study on the Metabolism of Jujuboside A by Rat's Stomach Contents in Vitro with HPLC-MS/MS

作　　者：马桂劼 张妍[1] 张明春[1,2] 解军波[1,2] 庞广昌[1,2] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津市食品生物技术重点实验室,天津300134

出　　处：《食品研究与开发》2013年第19期1-4,共4页Food Research and Development

基　　金：国家自然科学基金资助项目(31000749)

摘　　要：采用HPLC-MS/MS法,研究酸枣仁皂苷A在体外模拟大鼠胃内容物中的代谢趋势及其代谢产物。收集Wistar大鼠胃内容物,于37℃下孵育一定浓度酸枣仁皂苷A,测定不同时间点样品中的酸枣仁皂苷A浓度,并分析其代谢规律及代谢产物的可能结构。结果表明:酸枣仁皂苷A在胃内容物中有明显降解趋势,6 h后降解百分数为55.63%。在样品中检测到3种代谢产物,主要为酸枣仁皂苷A的系列水解产物。The metabolic tendency of Jujuboside A and its metabolites in rat＇s stomach contents were investigated by HPLC-MS/MS method.The stomach contents were collected from Wistar rats.The certain content of Jujuboside A was cultivated under the constant temperature of 37 ℃.A series of degradation samples were assayed at different time.The degradation regularity and possible structures of metabolites were analyzed.The degradation tendency of Jujuboside A in stomach contents was obvious,and degradation percentage was 55.63 % in 6 h.Three metabolites were identified as the serial hydrolysis products of Jujuboside A.

关 键 词：酸枣仁皂苷A 胃内容物 HPLC-MS MS 代谢产物 

分 类 号：R285[医药卫生—中药学]