右旋美托咪啶对大鼠脑缺血再灌注损伤的保护作用及机制  

Protective Effect of Dexmedetomidine on Brain Ischemic Reperfusion Injury in Rat

作　　者：陈图锋[1] 黄婵燕[2] 方佳峰[1] 王钟兴[2] 

机构地区：[1]中山大学附属第三医院胃肠外科,广东广州510630 [2]中山大学附属第一医院麻醉科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2013年第6期851-855,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2010B031600206;2010B060900030)

摘　　要：【目的】探讨右旋美托咪啶在脑缺血再灌注损伤中的保护作用和相关机制。【方法】42只雄性SD大鼠随机分为3组,每组14只:右旋美托咪啶组(Dex)采用右旋美托咪啶5μg·kg-1·h-1恒速输注2 h;拮抗剂组(Dex+Yoh)于开启注射泵前10 min经尾静脉输注拮抗剂育亨宾1 mg/kg,余用法同上组;对照组(Con)以同等速度输注生理盐水2 h。所有大鼠行大脑中动脉栓塞术,缺血90 min后再灌注。观察术后24 h神经功能评分、脑梗死体积、水肿指数以及脑组织白介素(IL)-1β、肿瘤坏死因子(TNF)-α水平、核蛋白NF-κB p65表达量。【结果】再灌注24 h后,脑梗死体积的比较显示Dex组(17±2.8)%较Dex+Yoh组(38±4.9)%与Con组(42±5.7)%明显减小(P<0.05),水肿指数比较显示Dex组(1.021±0.098)较Dex+Yoh组(1.342±0.137)与Con组(1.417±0.105)显著降低,差异有统计学意义(P<0.05)。脑组织IL-1β、TNF-α及NF-κB p65蛋白表达量的比较均显示Dex组较Dex+Yoh组与Con组明显下降(P<0.05)。【结论】右旋美托咪啶通过抑制NF-κB通路的激活减少促炎因子的产生,对大鼠局灶性脑缺血再灌注损伤具有保护作用。[Objective] To investigate the protective effect and mechanism of dexmedetomidine on the brain ischemic-reperfusion injury in rat.[Methods] Male Sprague-Dawley rats weighing 270-300 g were randomly divided into three groups （n =14 each group）：（1）dexmedetomidine group （Group Dex） received dexmedetomidine administration at a rate of 5 μg/kg/h for 2 h,（2） antagonist group （Group Dex＋Yoh） was given yohimbine 1 mg/kg 10 min before the same dexmedetomidine administration as Group Dex and （3）control group （Group Con） received 0.9% sodium chloride at a corresponding rate as placebo.All rats were subjected to right middle cerebral arterial occlusion （MCAO） for 90 min.After allowing reperfusion for 24 h,the cerebral infarction volume,edema index and neurological scores were measured,the levels of interleukin （IL）-1β,tumor necrosis factor （TNF）-α and the expression of nuclear NF-κB p65 in brain tissue were evaluated.[Results] After brain reperfusion for 24 hours,the infarction volume of Group Dex （17 ± 2.8）% was significantly lower than that of Group Dex＋Yoh （38 ± 4.9）% and Group Con （42 ± 5.7）% （P〈 0.05）,the edema index in Group Dex （1.021 ± 0.098） was decreased than that in Group Dex＋Yoh （1.342 ± 0.137） and Group Con （1.417 ± 0.105,P 〈 0.05）.Simultaneously,compared with Group Dex＋Yoh or Group Con,the tissue levels of IL-1β,TNF-α,and the expression of nuclear NF-κB p65 were significantly reduced in Group Dex （all P 〈 0.05）.[Conclusions] Dexmedetomidine can attenuate brain ischemic-reperfusion injury in rats partly through inhibiting the activation of NF-κB and suppressing the overexpression of pro-inflammatory factors.

关 键 词：右旋美托咪啶 脑 缺血再灌注 大鼠 

分 类 号：R651.1[医药卫生—外科学]