慢性阻塞性肺疾病加重期和稳定期Th17和Treg细胞的表达  

Expression of Th17 Cells and Tregs in SCOPD and AECOPD

作　　者：陈钢[1] 谢灿茂[1] 罗志扬[2] 李敏菁[2] 张培芳[2] 

机构地区：[1]中山大学附属第一医院呼吸内科,广东广州510080 [2]佛山市第一人民医院呼吸内科,广东佛山528000

出　　处：《中山大学学报（医学科学版）》2013年第6期917-921,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：佛山市科技局医学类科技攻关项目(201108076)

摘　　要：【目的】探讨慢性阻塞性肺疾病(COPD)急性加重期、稳定期患者和肺功能正常吸烟者外周血中Th17、Treg细胞的表达。【方法】选取慢性阻塞性肺疾病急性加重患者28例、慢性阻塞性肺疾病稳定期患者22例、吸烟肺功能正常者(对照组)22例为研究对象。流式细胞术检测外周血中Th17细胞、CD4+CD25highTreg细胞的比例,ELISA检测诱导痰IL-17、TGF-β1的水平。【结果】急性加重期COPD组的外周血Th17比例(%)较稳定期COPD组增高(2.92±0.16 vs 1.25±0.14,P<0.001)、痰液IL-17水平(ng/L)较稳定期COPD组增高(82.33±17.38 vs 12.64±2.74,P<0.001),外周血Treg比例(%)低于稳定期COPD组(8.52±0.47 vs 11.45±0.78,P<0.001)。急性加重期COPD组、稳定期COPD组外周血中Th17比例与Treg比例均呈负相关(r分别为-0.723和-0.674,P均<0.001)。【结论】急性加重期COPD的气道局部和全身的免疫反应以促炎的"Th17反应"占优势,稳定期COPD以"Treg反应"占优势。[Objective] To investigate the expression of Th17 cells and Tregs in peripheral blood from the patients of stable chronic obstructive pulmonary disease （SCOPD） and chronic obstructive pulmonary disease （AECOPD）.[Methods] 28 patients of AECOPD,22 outpatients of SCOPD and 22 smoking healthy donors were recruited.Th17 cells and CD4＋CD25high Tregs were determined by flow cytometry.Concentrations of IL-17 and TGF-β in the sputum were detected by ELISA.[Results] Percentages of peripheral Th 17 cells （2.92 ± 0.16%） in AECOPD were significantly higher than those in SCOPD （1.25 ± 0.14%,P 〈 0.001）,Level of IL-17 in sputum （82.33 ± 17.38 ng/L） in AECOPD was significantly higher than those in SCOPD （12.64 ± 2.74 ng/L,P〈0.001）.Percentages of peripheral Treg cells （8.52 ± 0.47%） in AECOPD were significantly lower than those in SCOPD （11.45 ± 0.78%,P 〈 0.001）.Remarkably negative correlations were seen between numbers of Th17 cells and Tregs in AECOPD （r =-0.723,P 〈 0.001）and SCOPD（r =-0.647,P 〈 0.001）.[Conclusions] Th17 response was dominated in AECOPD whereas Treg response was dominated in SCOPD.

关 键 词：慢性阻塞性肺疾病 TH17细胞 IL-17 调节性T细胞 

分 类 号：R563[医药卫生—呼吸系统]